Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study.
Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study.
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DOI：
10.1186/1472-6785-10-3
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发表时间：
2010-02-01
期刊：
影响因子：
--
通讯作者：
Hoikkala A
中科院分区：
文献类型：
--
作者：
Kankare M;Salminen T;Laiho A;Vesala L;Hoikkala A
昆虫滞育是一个重要的生物学过程，它涉及到个体和种群水平上对生存和生殖适合性至关重要的许多生活史参数。蒙大拿果蝇是华氏果蝇属的一种，具有深刻的光周期生殖滞育，使成虫能够在高纬度和高海拔的严酷冬季条件下生存。我们为蒙大拿果蝇创建了一个定制的微阵列，其中包含101个已知影响滞育、光周期、生殖行为、生物钟和应激耐受性的重要特征的基因。这个阵列让我们有机会筛选出在光周期生殖滞育期间表现出表达变化的基因，这些基因适应了生活在北纬地区的物种，环境条件的季节性变化很高。比较滞育、繁殖和幼龄蒙大拿雌虫，在芯片上发现了24个基因的表达变化；例如，在滞育和繁殖雌性之间的比较中，1个基因(果蝇冷适应基因，DCA)在滞育雌性中表达上调，15个基因在滞育雌性中表达下调。其中7个基因的表达下调与滞育状态有关，而在5个基因中，表达的变化与雌性的年龄有关，而不是与它们的生殖状态有关。此外，qRT-PCR实验也证实了沙发薯(CPO)基因与蒙大拿的滞育有关。事实证明，候选基因芯片提供了一种实用且经济高效的方法来追踪可能在光周期生殖滞育以及进一步适应季节变化的环境条件中发挥重要作用的基因。本研究揭示了DCA和CPO两个基因，它们在蒙大拿光周期滞育中的作用值得更详细地研究。此外，利用候选基因微阵列进行更具体的实验设计和目标组织的进一步研究，可能会发现更多具有更多受限表达模式的基因。
Insect diapause is an important biological process which involves many life-history parameters important for survival and reproductive fitness at both individual and population level. Drosophila montana, a species of D. virilis group, has a profound photoperiodic reproductive diapause that enables the adult flies to survive through the harsh winter conditions of high latitudes and altitudes. We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes in environmental conditions. Comparisons among diapausing, reproducing and young D. montana females revealed expression changes in 24 genes on microarray; for example in comparison between diapausing and reproducing females one gene (Drosophila cold acclimation gene, Dca) showed up-regulation and 15 genes showed down-regulation in diapausing females. Down-regulation of seven of these genes was specific to diapause state while in five genes the expression changes were linked with the age of the females rather than with their reproductive status. Also, qRT-PCR experiments confirmed couch potato (cpo) gene to be involved in diapause of D. montana. A candidate gene microarray proved to offer a practical and cost-effective way to trace genes that are likely to play an important role in photoperiodic reproductive diapause and further in adaptation to seasonally varying environmental conditions. The present study revealed two genes, Dca and cpo, whose role in photoperiodic diapause in D. montana is worth of studying in more details. Also, further studies using the candidate gene microarray with more specific experimental designs and target tissues may reveal additional genes with more restricted expression patterns.
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影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
10.7
作者：
Nuzhdin, SV;Wayne, ML;McIntyre, LM
通讯作者：
McIntyre, LM
影响因子：
3.5
作者：
Goto, SG;Kimura, MT
通讯作者：
Kimura, MT
DOI：
10.1006/bbrc.1998.9327
发表时间：
1998-09-18
影响因子：
3.1
作者：
Fujita, T;Inoue, H;Maruyama, N
通讯作者：
Maruyama, N
影响因子：
4.9
作者：
Mirol, P. M.;Schaefer, M. A.;Butlin, R. K.
通讯作者：
Butlin, R. K.