In vitro study of the age-dependent caecal fermentation pattern and methanogenesis in young rabbits.
In vitro study of the age-dependent caecal fermentation pattern and methanogenesis in young rabbits.
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幼兔年龄依赖性盲肠发酵模式和产甲烷作用的体外研究。
DOI：
10.1051/rnd:19960303
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
L. Maertens
中科院分区：
文献类型：
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作者：
F. Piattoni;D. Demeyer;L. Maertens
盲肠发酵模式，包括产甲烷，在年轻的兔子进行了研究，使用在体外分批孵育。使用6窝常规窝，每窝8只家兔。在22、25、28、32、36、42和56日龄时，从每窝中屠宰一只动物，并将其盲肠内容物用于39 ℃/24 h的体外分批孵育。分析孵育样品的挥发性脂肪酸（VFA）、甲烷、氢气、氨氮（NH3-N）和乳酸（LA）。尽管在第36天观察到显著降低，但净体外VFA总产量随年龄的变化没有明显差异，反映了畜牧业性能的降低可能与同一时期发生的螺形梭菌感染有关。丁酸盐和丙酸盐的摩尔比例随着年龄的增长形成了相反方向的变化，在第25天从丙酸盐突然转变为丁酸盐。在体外NH3-N的生产是一个渐进的和显着的下降，随着年龄的增长，在体外LA生产总是很低。甲烷生产几乎没有发酵，直到32天的年龄，之后突然从1.6到52.0 μ mol/瓶/天，并进一步增加与年龄。一个显着的窝产甲烷的影响进行了观察，这表明存在的遗传效应。产氢量相当低，从第36天开始随着产甲烷量的增加而显著降低。计算的氢气回收率显示从第32天开始逐渐增加，并且与甲烷产量呈正相关（r = 0.92）。总之，它似乎是在年轻的哺乳兔，减少产乙酸是盲肠发酵的主要特点，逐渐和部分取代产甲烷与固体饲料摄入量的增加。
The caecal fermentation pattern, including methanogenesis, was studied in young rabbits using in vitro batch incubations. Six conventional litters of eight rabbits each were used. At the age of 22, 25, 28, 32, 36, 42 and 56 days, an animal was slaughtered from each litter and its caecal contents were used for in vitro batch incubations at 39 degrees C/24 h. The incubated samples were analysed for volatile fatty acids (VFA), methane, hydrogen, ammonia nitrogen (NH3-N) and lactic acid (LA). The net total in vitro VFA production did not differ clearly with age, although a significant decrease was observed on day 36, reflecting the reduced zootechnical performances probably related to an infection with Clostridium spiroforme that occurred in the same period. The molar proportions of butyrate and propionate formed a change in the opposite direction with age, starting with a sudden shift from propionate to butyrate at day 25. In vitro NH3-N production was suggestive of a progressive and significant decrease with age; in vitro LA production was always low. Methane production was almost absent from fermentation until 32 days of age, after which it suddenly shifted from 1.6 to 52.0 mumol/flask/day and increased further with age. A significant litter effect on methanogenesis was observed which suggested the existence of a genetic effect. The hydrogen production was quite low and decreased significantly from day 36 with increasing methanogenesis. The calculated hydrogen recoveries showed a gradual increase from day 32 and were positively correlated (r = 0.92) with methane production. In conclusion, it would seem that in young suckling rabbits, reductive acetogenesis is a major characteristic of caecal fermentation, to be replaced gradually and partially by methanogenesis with the increasing intake of solid feed.