Combination effect of three anti-HSV-2 active plant extracts exhibiting different modes of action
Combination effect of three anti-HSV-2 active plant extracts exhibiting different modes of action
复制标题
DOI：
10.1007/s13596-020-00430-0
复制
发表时间：
2020-06-01
影响因子：
2
通讯作者：
Bouslama, Lamjed
中科院分区：
文献类型：
--
作者：
Benzekri, Roudaina;Limam, Ferid;Bouslama, Lamjed
多重耐药病毒的出现导致发现新的药物来源或克服这一障碍的新策略。联合草药疗法正在成为一种有前途的提高效率的方法。在这项研究中，研究了黄连木和骆驼蓬的抗 HSV-2 活性。分别使用 MTT 测试和空斑减少测定评估了来自不同器官的 28 种有机提取物对 Vero 细胞的细胞毒性及其抗 HSV-2 活性。骆驼蓬甲醇种子提取物、黄连木甲醇茎和二氯甲烷果实提取物对HSV-2有活性，选择性指数值分别为13.19、51.23和10.23。这些活性提取物表现出不同的作用模式：（1）骆驼蓬甲醇种子提取物在病毒吸附和渗透之前表现出杀病毒作用，并且在新形成的病毒粒子释放到感染细胞外之前表现出杀病毒作用-（2）黄连木甲醇茎提取物仅在病毒吸附和渗透之前表现出杀病毒作用-（3）黄连木二氯甲烷果实提取物在感染后长达7小时表现出病毒抑制作用。三种活性提取物的二元组合并没有提高它们各自的活性。该报告有助于证明活性提取物的组合不一定会增加初始活性。
The emergence of multidrug-resistant viruses leads to discover a novel source of drugs or a new strategy to overcome this obstacle. Combined herbal therapies are becoming a promising approach for a better efficiency. In this study, Pistacia lentiscus and Peganum harmala were investigated for their anti HSV-2 activity. Twenty eight organic extracts from different organs were evaluated for their cytotoxicity on Vero cells and their anti HSV-2 activity using the MTT test and the plaque reduction assay, respectively. Peganum harmala methanol seeds extract, Pistacia lentiscus methanol stem and dichloromethane fruit extracts were active on HSV-2 with selectivity index values of 13.19, 51.23 and 10.23, respectively. These active extracts exhibited different mode of action: (1) Peganum harmala methanol seeds extract showed a virucidal action before virus adsorption and penetration, and on the newly formed virions before their release outside infected cells-(2) Pistacia lentiscus methanol stem extract exhibited virucidal effect only before virus adsorption and penetration-(3) Pistacia lentiscus dichloromethane fruit extracts demonstrated virus inhibition up to 7 h post infection. The binary combination of the three active extracts did not improve their individually activity. This report could contribute to prove that combination of active extracts do not necessary increase the initial activity.