Potential Use of CRISPR/Cas13 Machinery in Understanding Virus-Host Interaction.
Potential Use of CRISPR/Cas13 Machinery in Understanding Virus-Host Interaction.
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DOI：
10.3389/fmicb.2021.743580
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发表时间：
2021
影响因子：
5.2
通讯作者：
Munir M
中科院分区：
文献类型：
--
作者：
Bayoumi M;Munir M
原核生物在进化过程中获得了免疫系统，以抵御入侵的移动的遗传因子，包括病毒和质粒。通过识别入侵核酸的特定序列，原核生物介导随后的降解过程，统称为规则间隔短回文重复序列（CRISPR）-CRISPR相关（Cas）（CRISPR-Cas）系统。CRISPR-Cas系统根据效应Cas蛋白的结构分为两大类。I类系统具有由多个蛋白质组成的效应子模块，而II类系统具有单个多结构域效应子。此外，CRISPR-Cas系统还可以根据间隔区获取组分及其进化特征分类为类型，即I-VI型。在CRISPR/Cas系统中，Cas9是识别、降解和调节DNA的最常见的多结构域核酸酶之一。重要的是，最近发现Cas蛋白的变体靶向RNA，特别是单效应Cas 13核酸酶。Cas 13彻底改变了我们在内源性微环境中研究和干扰RNA的能力。Cas 13效应子为在病毒学和生物技术领域开发新的研究工具提供了一个很好的候选者。在本文中，我们的目的是提供Cas 13靶向病毒RNA用于RNA介导的降解或基于CRISPR-Cas 13的诊断的最新进展的全面总结。此外，我们的目标是提供一个概述的拟议的应用程序，可以彻底改变我们的理解病毒宿主相互作用使用Cas 13介导的方法。
Prokaryotes have evolutionarily acquired an immune system to fend off invading mobile genetic elements, including viral phages and plasmids. Through recognizing specific sequences of the invading nucleic acid, prokaryotes mediate a subsequent degradation process collectively referred to as the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)–CRISPR-associated (Cas) (CRISPR–Cas) system. The CRISPR–Cas systems are divided into two main classes depending on the structure of the effector Cas proteins. Class I systems have effector modules consisting of multiple proteins, while class II systems have a single multidomain effector. Additionally, the CRISPR–Cas systems can also be categorized into types depending on the spacer acquisition components and their evolutionary features, namely, types I–VI. Among CRISPR/Cas systems, Cas9 is one of the most common multidomain nucleases that identify, degrade, and modulate DNA. Importantly, variants of Cas proteins have recently been found to target RNA, especially the single-effector Cas13 nucleases. The Cas13 has revolutionized our ability to study and perturb RNAs in endogenous microenvironments. The Cas13 effectors offer an excellent candidate for developing novel research tools in virological and biotechnological fields. Herein, in this review, we aim to provide a comprehensive summary of the recent advances of Cas13s for targeting viral RNA for either RNA-mediated degradation or CRISPR–Cas13-based diagnostics. Additionally, we aim to provide an overview of the proposed applications that could revolutionize our understanding of viral–host interactions using Cas13-mediated approaches.
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影响因子：
4.7
作者：
Aman, Rashid;Mahas, Ahmed;Mahfouz, Magdy
通讯作者：
Mahfouz, Magdy
DOI：
10.1126/science.aaf5573
发表时间：
2016-08-05
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
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通讯作者：
Zhang F
影响因子：
5.5
作者：
Bayoumi M;Munir M
通讯作者：
Munir M
影响因子：
64.8
作者：
Ackerman, Cheri M.;Myhrvold, Cameron;Sabeti, Pardis C.
通讯作者：
Sabeti, Pardis C.
影响因子：
16.6
作者：
Barnes, Kayla G.;Lachenauer, Anna E.;Sabeti, Pardis C.
通讯作者：
Sabeti, Pardis C.