Impacts of Early Life Exposure to Estrogen on Subsequent Breeding Behavior and Reproductive Success in Zebrafish
Impacts of Early Life Exposure to Estrogen on Subsequent Breeding Behavior and Reproductive Success in Zebrafish
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DOI：
10.1021/es101185b
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发表时间：
2010-08-15
影响因子：
11.4
通讯作者：
Tyler, Charles R.
中科院分区：
文献类型：
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作者：
Coe, Tobias S.;Soeffker, Marta K.;Tyler, Charles R.
暴露于环境雌激素对生殖发育的影响已有充分记录，但最近引起了更广泛的关注，因为有证据表明，这种暴露可能会破坏生殖行为、优势和亲子关系的正常模式，并可能对人口水平产生影响。因此，了解任何此类影响对于有效的风险评估至关重要。本研究调查了生命早期（受精后 20-60 天）短暂暴露于炔雌醇 (EE2)（包括环境范围内的剂量水平）对雄性和雌性斑马鱼 (Danio rerio) 在竞争性繁殖场景中随后的繁殖行为和成功的影响。生命早期接触 EE2 对成熟男性或女性随后的性腺表型没有明显影响。事实上，在竞争性产卵场景中，暴露于 2.76 ng EE2/L 的雄性的繁殖成功率有所提高。相比之下，雌性在生命早期接触 EE2 (9.86 ng/L) 会降低其随后在竞争性产卵场景中的繁殖成功率。择偶实验表明，这是雌性对雄性求爱行为减少的结果，而不是雄性对未接触过的雌性的偏爱。雌性的生殖能力通常被认为是种群人口统计和动态的关键决定因素，因此暴露于 EE2 对雌性生殖成功的影响可能对暴露的鱼类种群产生重大影响。
Impacts of exposure to environmental estrogens on reproductive development are well documented, but recently wider concern has been raised due to evidence that such exposures can disrupt normal patterns of reproductive behavior, dominance, and parentage, with potential population level implications. It is fundamental therefore to understand any such effects for effective risk assessment. This study investigated the impact of a transient exposure to ethinylestradiol (EE2) during early life (from 20-60 days post fertilization), including at a dosing level within the environmental range, on the subsequent reproductive behavior and success in both male and female zebrafish (Danio rerio) in competitive breeding scenarios. There were no obvious effects of the early life EE2 exposures on the subsequent gonadal phenotypes in either mature males or females. In fact, reproductive success in males exposed to 2.76 ng EE2/L was increased in competitive spawning scenarios. In contrast, exposure of females to EE2 (9.86 ng/L) during early life reduced their subsequent reproductive success in competitive spawning scenarios. Mate choice experiments suggested this was a consequence of the females' diminished courting behavior toward males, rather than any male preference for unexposed females. Reproductive capability of females is generally considered a key determinant in population demographics and dynamics, and therefore the effect of exposure to EE2 on female reproductive success may have significant implications for exposed fish populations.