Ratiometric Luminescent Detection of Bacterial Spores with Terbium Chelated Semiconducting Polymer Dots
Ratiometric Luminescent Detection of Bacterial Spores with Terbium Chelated Semiconducting Polymer Dots
复制标题
铽螯合半导体聚合物点比率发光检测细菌孢子
DOI：
10.1021/ac4016616
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发表时间：
2013-10-01
影响因子：
7.4
通讯作者：
Qin, Weiping
中科院分区：
文献类型：
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作者：
Li, Qiong;Sun, Kai;Qin, Weiping
我们报道了一种基于铽离子螯合的半导体聚合物点的比率荧光传感器，用于检测水溶液中的细菌孢子。荧光聚芴（PFO）点作为一个支架，以配合镧系离子，可以敏化吡啶二羧酸钙（CaDPA），细菌孢子的一个重要的生物标志物。PFO点的吸收带延伸到深紫外区，允许参比和敏化剂都可以用单一波长（类似于275 nm）激发。PFO的荧光作为参考保持恒定，而Tb 3+离子在与DPA结合后表现出增强的发光。β相PFO点的尖锐荧光峰和Tb3+离子的窄带发射使得能够进行比率和灵敏的CaDPA检测，其在纳摩尔浓度上具有线性响应，并且检测限类似于0.2 nM。基于Pdots的传感器还显示出对CaDPA优于其他芳族配体的优异选择性。我们的研究结果表明，Tb 3+螯合Pdots传感器是有前途的灵敏和快速检测细菌孢子。
We report a ratiometric fluorescent sensor based on semiconducting polymer dots chelated with terbium ions to detect bacterial spores in aqueous solution. Fluorescent polyfluorene (PFO) dots serve as a scaffold to coordinate with lanthanide ions that can be sensitized by calcium dipicolinate (CaDPA), an important biomarker of bacterial spores. The absorption band of PFO dots extends to deep UV region, allowing both the reference and the sensitizer can be excited with a single wavelength (similar to 275 nm). The fluorescence of PFO remains constant as a reference, while the Tb3+ ions exhibit enhanced luminescence upon binding with DPA. The sharp fluorescence peaks of beta-phase PFO dots and the narrow-band emissions of Tb3+ ions enable ratiometric and sensitive CaDPA detection with a linear response over nanomolar concentration and a detection limit of similar to 0.2 nM. The Pdots based sensor also show excellent selectivity to CaDPA over other aromatic ligands. Our results indicate that the Tb3+ chelated Pdots sensor is promising for sensitive and rapid detection of bacterial spores.