Effects of catecholamines on fetal rat cardiocytes in vitro.
Effects of catecholamines on fetal rat cardiocytes in vitro.
复制标题
儿茶酚胺对体外胎鼠心肌细胞的影响。
DOI：
10.1002/aja.1001860203
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Mercer,WE
中科院分区：
文献类型：
--
作者：
Marino,TA;Walter,RA;D'Ambra,K;Mercer,WE
去甲肾上腺素能刺激未分裂的新生儿心肌细胞的大小增长，并能刺激培养中分裂的肝细胞和内皮细胞的数量增长。本研究的目的是验证一个假设，即在分裂胎儿心脏细胞时，去甲肾上腺素会刺激细胞数量而不是细胞大小的增长。将14天的胎儿心脏细胞置于无血清或补血清培养基中，分别在去甲肾上腺素（NE）、去甲肾上腺素加心得安或异丙肾上腺素存在或不存在的情况下培养4天。在此期间，通过增殖细胞核抗原（PCNA）抗体染色确定，在血清补充培养基中，几乎90%的心肌细胞处于细胞周期。此外，在培养的第2天和第4天，35%和40%的这些心肌细胞被3h‐胸腺嘧啶标记。4天后，与无血清培养相比，血清添加NE培养的心肌细胞数量增加。相比之下，各组之间的心肌细胞体积没有明显变化。结果表明，去甲肾上腺素对肌肉细胞群分化的作用是促进细胞增殖，而不是刺激细胞大小的增长。
Norepinephrine stimulates the growth in size of non‐dividing, neonatal cardiac muscle cells, and it can stimulate the growth in numbers of dividing hepatocytes and endothelial cells in culture. The objective of this study was to test the hypothesis that in dividing fetal cardiocytes, norepinephrine would stimulate growth in cell number rather than in cell size. Fourteen‐day fetal heart cells were placed in serum‐free or serum‐supplemented cultures in the presence or absence of norepinephrine (NE), NE plus propranolol, or isoproterenol for 4 days. Almost 90% of the cardiocytes in serum‐supplemented medium were in the cell cycle as determined by proliferating cell nuclear antigen (PCNA) antibody staining during this period. In addition, between days 2 and 4 of culture, 35% and 40% of these cardiocytes were labeled with3H‐thymidine. After 4 days the cardiocytes increased in cell number in the serum‐supplemented NE cultures as compared to serum‐free cultures. In contrast, there was no significant change in cardiocyte volume between any of the groups examined. It was concluded that in dividing muscle cell populations the effect of norepinephrine was to enhance cell proliferation rather than to stimulate cell growth in size.