CHROMOSOMAL LOCATION OF THE GENES FOR HUMAN-IMMUNOGLOBULIN HEAVY-CHAINS
CHROMOSOMAL LOCATION OF THE GENES FOR HUMAN-IMMUNOGLOBULIN HEAVY-CHAINS
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DOI：
10.1073/pnas.76.7.3416
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发表时间：
1979-01-01
影响因子：
11.1
通讯作者：
KOPROWSKI, H
中科院分区：
文献类型：
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作者：
CROCE, CM;SHANDER, M;KOPROWSKI, H
研究了次黄嘌呤磷酸核糖基转移酶（EC 2.4.2.8）缺陷的P3 x 63 Ag 8小鼠骨髓瘤细胞与人外周淋巴细胞或人淋巴母细胞或骨髓瘤细胞之间的体细胞杂交体，以产生人免疫球蛋白[IG]链和表达分配给每个不同人染色体的酶标记物。人染色体14是存在于所有产生μ的独立杂交体中的唯一人染色体，γ。和α。人类H链。在2个产生人类H链的独立杂交细胞中，14号染色体是杂交细胞中唯一存在的人类染色体。这些杂交体中人14号染色体的丢失导致其产生人IG H链的能力的同时丧失。人IG H链的基因显然位于产生Ig的人细胞中的人染色体14上。
Somatic cell hybrids between P3x63Ag8 mouse myeloma cells deficient in hypoxanthine phosphoribosyltransferase (EC 2.4.2.8) and either human peripheral lymphocytes or human lymphoblastoid or myeloma cells were studied for the production of human immunoglobulin [Ig] chains and for the expression of enzyme markers assigned to each of the different human chromosomes. Human chromosome 14 was the only human chromosome present in all independent hybrids producing .mu., .gamma. and .alpha. human H chains. In 2 of the independent hybrids that produced human H chains, chromosome 14 was the only human chromosome present in the hybrid cells. Loss of human chromosome 14 from these hybrids resulted in the concomitant loss of their ability to produce human Ig H chains. The genes for human Ig H chains are apparently located on human chromosome 14 in Ig-producing human cells.