High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate flourescence reader in 96-well format
High-throughput assay of oxygen radical absorbance capacity (ORAC) using a multichannel liquid handling system coupled with a microplate flourescence reader in 96-well format
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DOI：
10.1021/jf0201529
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发表时间：
2002-07-31
影响因子：
6.1
通讯作者：
Prior, RL
中科院分区：
文献类型：
--
作者：
Huang, DJ;Ou, BX;Prior, RL
氧自由基吸收能力（ORAC）测定法已被广泛接受，作为一种标准的工具来衡量，在营养品，制药和食品工业的抗氧化活性。然而，由于COBAS FARA II分析仪（制造商停产的仪器）不可用，ORAC测定因缺乏可访问性而受到批评。此外，手动样品制备耗时且劳动强度大。本研究的目的是开发一种高通量仪器平台，可以完全自动化ORAC测定程序。新的仪器平台包括一个机器人八通道液体处理系统和一个微孔板荧光阅读器。通过使用高通量平台，提高了测定的效率，样品通量比当前程序增加至少10倍。日内和日间CV的平均值小于或等于15%，检测限和定量限分别为5和6.25 μ M。
The oxygen radical absorbance capacity (ORAC) assay has-been widely accepted as a standard tool to measure the, antioxidant activity in the nutraceutical, pharmaceutical, and food industries. However, the ORAC assay has been criticized for a lack of accessibility due to the unavailability of the COBAS FARA II analyzer, an instrument discontinued by the manufacturer. In addition, the manual sample preparation is time-consuming and labor-intensive. The objective of this study was to develop a high-throughput instrument platform that can fully automate the ORAC assay procedure. The new instrument platform consists of a robotic eight-channel liquid handling system and a microplate fluorescence reader. By using the high-throughput platform, the efficiency of the assay is improved with at least a 10-fold increase in sample throughput over the current procedure. The mean of intra- and interday CVs was less than or equal to15%, and the limit of detection and limit of quantitation were 5 and 6.25 muM, respectively.