Lipidomics: An analysis of cellular lipids by ESI-MS
Lipidomics: An analysis of cellular lipids by ESI-MS
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DOI：
10.1016/j.ymeth.2006.05.014
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发表时间：
2006-06-01
期刊：
影响因子：
4.8
通讯作者：
Brown, H. Alex
中科院分区：
文献类型：
--
作者：
Milne, Stephen;Ivanova, Pavlina;Brown, H. Alex
对脂质信号在细胞功能中的重要性的认识导致了脂质分析技术的快速发展。对脂类变化的测量是确定细胞信号网络(例如，激素或药物激活受体)的核心，而脂类参与了许多生化和病理过程。在过去的几年里，我们实验室致力于开发从生物系统中提取甘油磷脂的有效方法，并利用质谱仪对其进行检测和鉴定。我们分析了哺乳动物细胞中磷脂的变化，作为明确的配体刺激策略的结果，这支持了该联盟的研究问题。改进了用于磷脂分析的质谱学技术，结合我们小组开发的复杂的计算方法，促进了对哺乳动物细胞中数百种磷脂的同时分析。该信息以脂质阵列(或者更准确地说是虚拟阵列)的形式呈现，并且允许识别两种不同的生物条件(例如，未刺激的基础状态与刺激的基础状态，或者作为正常和疾病阶段之间的对比)之间的膜磷脂物种的时间变化。利用脂质组学的方法，我们能够从总的膜提取物中鉴定出大约450种磷脂，并定性地测量由细胞表面受体引发的模式反应变化。因此，这种方法有助于阐明由细胞内的扰动引起的代谢变化，并有助于识别信号模式。(C)2006 Elsevier Inc.保留所有权利。
Recognition of the importance of lipid signaling in cellular function has led to rapid progress in the technology of lipid analysis. Measurements of lipid species changes are central to defining the networks of cell signaling (e.g., receptor activation by hormones or drugs) and lipids are involved in many biochemical and pathological processes. During the last several years our laboratory has focused on developing efficient methods for extraction of glycerophospholipids from biological systems and their detection and identification by mass spectrometry. We analyze phospholipid changes in mammalian cells as a result of a defined ligand stimulation strategy that supports the research questions of the consortium. The improvement of mass spectrometry techniques for phospholipid analysis combined with sophisticated computational methods developed in our group has facilitated simultaneous analysis of hundreds of phospholipid species in mammalian cells. This information is presented as Lipid Arrays (or more precisely as virtual arrays) and allows identification of temporal changes in membrane phospholipid species between two contrasting biological conditions (e.g., unstimulated basal vs. stimulated or as a contrast between normal and disease stages). Using the lipidomics approach, we are able to identify approximately 450 phospholipid species from total membrane extracts and qualitatively measure pattern response changes initiated by cell surface receptors. As such, this approach facilitates the elucidation of the metabolic changes induced by a perturbation in the cell and recognition of patterns of signaling. (c) 2006 Elsevier Inc. All rights reserved.