Principles of the xTAG™ respiratory viral panel assay (RVP Assay)
Principles of the xTAG™ respiratory viral panel assay (RVP Assay)
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DOI：
10.1016/s1386-6532(07)70007-0
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发表时间：
2007-11-01
影响因子：
8.8
通讯作者：
Janeczko, Richard
中科院分区：
文献类型：
--
作者：
Merante, Frank;Yaghoubian, Sevana;Janeczko, Richard
1.背景多重核酸检测能够同时检测多种分析物，并使每个结果的成本最小化，同时保持高的分析和临床灵敏度和特异性。基于Luminex微珠微阵列的通用阵列平台为基因分型和基于传染病的检测提供了这些理想的特性。该平台利用由100个光谱上不同的珠子组成的经验证的阵列，每个珠子与包含Luminex Universal阵列的独特寡核苷酸引物序列偶联（Bortolin等人，2004年）。该阵列用于临床基因分型的用途已经有很好的文献记载（Bortolin等人，2004; Strom等人，2005年; Zhu等人，2007）并且最近已经举例说明了其用于传染病测试的效用（Mahony等人，2007年）。通常，基因分型测定需要扩增和询问测试中存在的所有等位基因。另一方面，传染病检测，如Luminex xTAG ™ RVP测定的情况，通常检测临床样品中存在的一种或多种靶序列的存在。与目前依赖于培养和荧光技术的传染病诊断实验室标准相比，xTAGTM RVP检测试剂盒为临床医生提供了检测呼吸道病毒的新工具，具有更高的灵敏度和特异性，并提高了阳性和阴性预测值。通过在同一检测中提供更广泛的靶覆盖，多重方法消除了当症状性患者通过不太全面的方法检测为阴性时发生的诊断疑问。Luminex Molecular Diagnostics（LMD）呼吸道病毒检测试剂盒（xTAG？RVP）是一种多重核酸检测试剂盒，可检测几乎所有与呼吸道或流感样疾病相关的病毒和某些亚型。它基于LMD专有的通用阵列平台，可以检测20种不同的呼吸道病毒和亚型
1. BackgroundMultiplexed nucleic assays enable the simultaneous detection of multiple analytes and minimize the cost per result while maintaining high analytical and clinical sensitivity and specificity. The Luminex bead microarray based Universal array platform delivers on these desirable characteristics for both genotyping and infectious disease based assays. The platform utilizes a validated array comprised of 100 spectrally distinct beads, each of which is coupled to a unique oligonucleotide primer sequence comprising the Luminex Universal array (Bortolin et al., 2004). The use of this array for clinical genotyping has been well documented (Bortolin et al., 2004; Strom et al., 2005; Zhu et al., 2007) and most recently its utility for infectious disease testing has been exemplified (Mahony et al., 2007). Generally genotyping assays require the amplification and interrogation of all alleles present in the test. Infectious disease testing on the other hand, as is the case for the Luminex xTAGTM RVP assay, generally detects the presence of one or more target sequences when present in a clinical sample. The xTAGTM RVP assay provides clinicians with new tools with greater sensitivity and specificity and improved positive and negative predictive values in detecting respiratory viruses compared to current infectious disease diagnostic laboratory standards which rely upon culture and fluorescence techniques. By providing broader target coverage in the same assay, multiplexing methods obviate the diagnostic doubt that occurs when a symptomatic patient tests negative by less comprehensive methods. The Luminex Molecular Diagnostics (LMD) Respiratory Viral Panel (xTAGTM RVP) is a multiplexed nucleic acid assay covering almost all viruses and some subtypes associated with respiratory or influenza-like-illness. It is based on LMD’s proprietary universal array platform and allows detection of 20 distinct respiratory viruses and subtypes