Measurement and analysis of shape recovery process of each erythrocyte for estimation of its deformability by using micro-channel technique -Influence of softness of cell membrane and viscosity of hemoglobin solution inside cell-
Measurement and analysis of shape recovery process of each erythrocyte for estimation of its deformability by using micro-channel technique -Influence of softness of cell membrane and viscosity of hemoglobin solution inside cell-
复制标题
利用微通道技术测量和分析每个红细胞的形状恢复过程，以评估其变形能力 -细胞膜柔软度和细胞内血红蛋白溶液粘度的影响-
DOI：
10.1007/s12573-012-0054-7
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发表时间：
2013
影响因子：
--
通讯作者：
K. Ohba
中科院分区：
文献类型：
--
作者：
T. Tajikawa;Y. Imamura;T. Ohno;F. Muranishi;M. Kubota;K. Ohba
本研究试图通过测量红细胞离开微通道后的形状恢复时间常数来评估每个红细胞的变形能力。我们在PDMS片上制作了一个5μ m见方、100μ m长的微通道阵列。以健康红细胞为对照、人工膜硬化红细胞和人工血红蛋白溶液稀释红细胞三种不同的血液样品，研究红细胞力学性质变化对形状恢复时间常数的影响。采用标准的线性实体模型对形状恢复过程进行了建模和分析。结果表明，所有红细胞的压缩应变随时间的变化均呈一阶滞后系统的指数衰减规律，因此可以由半对数松弛曲线计算形状恢复的时间常数。与健康红细胞相比，使用戊二醛的细胞膜越硬，松弛时间常数越短。稀释的血红蛋白红细胞比健康的红细胞恢复得更快。此外，从女性采集的健康血液的时间常数明显短于从男性采集的健康血液。而血红蛋白完全替代红细胞的时间常数不受性别差异的影响。这些结果表明，无论个体和性别差异如何，健康细胞膜的硬度没有显著差异。另一方面，细胞内血红蛋白溶液的粘度是影响时间常数的重要因素之一。因此，这些结果表明，单个红细胞的变形性可以通过微通道技术测量的松弛时间常数来定量。
This study tried to evaluate the deformability of each erythrocyte by measuring the time constant of shape recovery just after the erythrocytes left the microchannels. We fabricated a microchannel array with a 5μm-square, 100μm-long cross-section on a PDMS sheet. Three different kinds of blood samples were prepared—healthy erythrocytes as a control, artificially membrane-hardened erythrocytes and artificial hemoglobin solution-diluted erythrocytes—to investigate the influence of erythrocyte's mechanical property changes on the time constant of shape recovery. These shape recovery processes were modeled and analyzed by a standard liner solid model. As a result, the temporal variation of the compressive strain of all erythrocytes showed exponential decay with time elapsed like a first order lag system, so the time constant of shape recovery could be calculated from the semi-logarithmic relaxation curve. The stiffer the cell membrane was using glutaraldehyde, the shorter the time constant for relaxation became compared to healthy erythrocytes. The diluted hemoglobin erythrocytes snapped back quicker than healthy ones. In addition, the time constant of healthy blood drawn from females was clearly shorter than that collected from males. However, the time constant of fully hemoglobin substituted erythrocytes was not affected by gender difference. These results indicate that there is not a significant difference in the stiffness of healthy cell membranes regardless of individual and gender differences. On the other hand, the viscosity of the hemoglobin solution inside the cell is one of the significant factors affecting the time constant. Therefore, these results suggest that the deformability of individual erythrocytes can be quantitated by the time constant for relaxation measured by microchannel techniques.