Rab proteins of Drosophila melanogaster: Novel members of the Rab‐protein family
Rab proteins of Drosophila melanogaster: Novel members of the Rab‐protein family
复制标题
黑腹果蝇的 Rab 蛋白：Rab 蛋白家族的新成员
DOI：
10.1016/s0014-5793(97)00094-x
复制
发表时间：
1997
期刊：
影响因子：
3.5
通讯作者：
K. Ozaki
中科院分区：
文献类型：
--
作者：
A. Satoh;F. Tokunaga;K. Ozaki
我们从果蝇头部cDNA文库中分离出9个cDNA克隆，每个克隆编码一个不同的rab蛋白家族成员。其中7个（DRabs）与哺乳动物Rab蛋白对应成员的氨基酸同源性超过80%。另外两个蛋白（DRabRP3和4）与已知的Rab蛋白序列相似性较低。然而，两者都含有已知Rabs中保守的所有氨基酸。此外，在大肠杆菌细胞中合成时，DRabRP4具有很强的gtp结合活性。这些结果表明，drabrp是rab蛋白家族的新成员。分子系统发育分析也支持这一结论。©1997欧洲生化学会联合会。
From a Drosophila head cDNA library, we isolated 9 cDNA clones, each of which encodes a different member of Rab-protein family. Seven of them (DRabs) have more than 80% amino acid identity to the corresponding members of mammalian Rab proteins. The other two proteins (DRabRP3 and 4) show low sequence similarity to any of the known Rab proteins. However, both contain all amino acids conserved in known Rabs. In addition, DRabRP4 has strong GTP-binding activity, when synthesized in E. coli cells. These results indicate that DRabRPs are novel members of the Rab-protein family. Molecular phylogenetic analysis also supported this conclusion. © 1997 Federation of European Biochemical Societies.