Comparison of the dynamics and functional redundancy of the arabidopsis dynamin-related isoforms DRP1A and DRP1C during plant development
Comparison of the dynamics and functional redundancy of the arabidopsis dynamin-related isoforms DRP1A and DRP1C during plant development
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DOI：
10.1104/pp.108.116863
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发表时间：
2008-08-01
期刊：
影响因子：
7.4
通讯作者：
Bednarek, Sebastian Y.
中科院分区：
文献类型：
--
作者：
Konopka, Catherine A.;Bednarek, Sebastian Y.
拟南芥（Arabidopsis thaliana）中的蛋白相关蛋白1（DRP1）家族成员是胞质分裂和细胞扩增所必需的。DRP1A和DRP1C两种亚型分别在柱头乳突扩展和花粉发育过程中维持质膜。目前还不清楚DRP1是否可互换功能，或者它们在细胞分裂和扩增过程中是否有不同的作用。DRP1C以前被证明在细胞皮层中形成动态焦点，其与植物中的网格蛋白内吞机制的一部分共定位。DRP1A定位于质膜，但其皮质组织和动力学尚未确定。使用双色标记与活细胞成像技术，我们表明，DRP1A也形成离散的动态焦点在表皮细胞皮层。尽管病灶与DRP1C和网格蛋白轻链形成的病灶重叠，但DRP1A和DRP1C病灶之间的行为和对药理学抑制剂的反应存在明显差异。DRP1A和DRP1C之间可能的功能或调节差异得到DRP1C在功能上补偿DRP1A缺失的失败的支持。我们的研究表明，DRP1亚型的功能或调节不同的细胞扩增过程中。
Members of the Arabidopsis ( Arabidopsis thaliana) DYNAMIN-RELATED PROTEIN1 (DRP1) family are required for cytokinesis and cell expansion. Two isoforms, DRP1A and DRP1C, are required for plasma membrane maintenance during stigmatic papillae expansion and pollen development, respectively. It is unknown whether the DRP1s function interchangeably or if they have distinct roles during cell division and expansion. DRP1C was previously shown to form dynamic foci in the cell cortex, which colocalize with part of the clathrin endocytic machinery in plants. DRP1A localizes to the plasma membrane, but its cortical organization and dynamics have not been determined. Using dual color labeling with live cell imaging techniques, we showed that DRP1A also forms discreet dynamic foci in the epidermal cell cortex. Although the foci overlap with those formed by DRP1C and clathrin light chain, there are clear differences in behavior and response to pharmacological inhibitors between DRP1A and DRP1C foci. Possible functional or regulatory differences between DRP1A and DRP1C were supported by the failure of DRP1C to functionally compensate for the absence of DRP1A. Our studies indicated that the DRP1 isoforms function or are regulated differently during cell expansion.