Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus.
Characterization and application of monoclonal antibodies against N protein of SARS-coronavirus.
复制标题
针对SARS-核负病毒N蛋白的单克隆抗体的表征和应用。
DOI：
10.1016/j.bbrc.2005.08.032
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发表时间：
2005-10-14
影响因子：
3.1
通讯作者：
Sun B
中科院分区：
文献类型：
--
作者：
Shang B;Wang XY;Yuan JW;Vabret A;Wu XD;Yang RF;Tian L;Ji YY;Deubel V;Sun B
严重急性呼吸道综合征冠状病毒（SARS-CoV）是通过呼吸道途径引起的一种传染病。早期有效、准确地诊断该病对于预防疾病传播和进行抗病毒治疗至关重要。本研究制备了抗SARS冠状病毒N蛋白的单克隆抗体，并利用不同截短的N蛋白片段进行了抗原表位的定位。这些表位的定位对构建用于血清学诊断的双抗体具有重要的意义。结果表明，所克隆的6株单抗均分为两组，分别识别249-317（A组）和317-395（B组）氨基酸序列。跨越氨基酸249-395的该区域含有位于N蛋白C-末端的主要B细胞表位。用SARS-CoV N蛋白特异性单克隆抗体和N蛋白特异性兔多克隆抗体组成双抗体夹心ELISA试剂盒。该试剂盒能够特异性地检测血清样品中的SARS-CoV N蛋白。
Severe acute respiratory syndrome-coronavirus (SARS-CoV) causes an infectious disease through respiratory route. Diagnosing the disease effectively and accurately at early stage is essential for preventing the disease transmission and performing antiviral treatment. In this study, we raised monoclonal antibodies (mAbs) against the nucleocapsid (N) protein of SARS-CoV and mapped epitopes by using different truncated N protein fragments. The mapping of those epitopes was valuable for constructing pair-Abs used in serological diagnosis. The results showed that all of the six raised mAbs were divided into two groups recognizing the region of amino acids 249–317 (A group) or 317–395 (B group). This region spanning amino acids 249–395 contains predominant B cell epitopes located at the C-terminus of N protein. One pair-Abs, consisting of N protein-specific rabbit polyclonal antibody and SARS-CoV N protein-specific mAb, was selected to construct a sandwich ELISA-kit. The kit was able to specifically detect SARS-CoV N proteins in serum samples.
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影响因子：
9.4
作者：
Che, XY;Qiu, LW;Yuen, KY
通讯作者：
Yuen, KY
影响因子：
4.4
作者：
Zeng, R;Ruan, HQ;Wu, JR
通讯作者：
Wu, JR
DOI：
10.1073/pnas.0306446101
发表时间：
2004-03-23
影响因子：
11.1
作者：
Simmons, G;Reeves, JD;Bates, P
通讯作者：
Bates, P
影响因子：
2.2
作者：
BEATTY, JD;BEATTY, BG;VLAHOS, WG
通讯作者：
VLAHOS, WG
影响因子：
11.8
作者：
Che XY;Hao W;Wang Y;Di B;Yin K;Xu YC;Feng CS;Wan ZY;Cheng VC;Yuen KY
通讯作者：
Yuen KY