Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
Estradiol-inducible AvrRps4 expression reveals distinct properties of TIR-NLR-mediated effector-triggered immunity
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DOI：
10.1093/jxb/erz571
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发表时间：
2020-03-25
影响因子：
6.9
通讯作者：
Jones, Jonathan D. G.
中科院分区：
文献类型：
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作者：
Ngou, Bruno Pok Man;Ahn, Hee Kyung;Jones, Jonathan D. G.
植物核苷酸结合区、富含亮氨酸重复序列的受体(NLR)蛋白在识别病原来源的效应物方面发挥着重要作用。然而，植物NLRs激活免疫的机制在很大程度上仍不清楚。对拟南芥NLRs RRS1-R和RPS4进行了很好的研究，这些NLRs可以识别细菌效应物AvrRps4和PopP2，但RRS1/RPS4复合体如何在效应器检测到后激活早期的即刻下游反应仍然知之甚少。为了在不受细胞表面受体免疫通路影响的情况下研究RRS1/RPS4的反应，我们建立了一个可诱导表达效应器AvrRps4的拟南芥品系。诱导不会导致过敏性细胞死亡反应(HR)，但会导致电解质渗漏，这通常与植物细胞死亡有关。在没有病原体的情况下，RRS1和RPS4的激活不能激活丝裂原相关蛋白激酶级联反应，但仍能激活防御基因的上调，从而对细菌产生抵抗力。
Plant nucleotide-binding domain, leucine-rich repeat receptor (NLR) proteins play important roles in recognition of pathogen-derived effectors. However, the mechanism by which plant NLRs activate immunity is still largely unknown. The paired Arabidopsis NLRs RRS1-R and RPS4, that confer recognition of bacterial effectors AvrRps4 and PopP2, are well studied, but how the RRS1/RPS4 complex activates early immediate downstream responses upon effector detection is still poorly understood. To study RRS1/RPS4 responses without the influence of cell surface receptor immune pathways, we generated an Arabidopsis line with inducible expression of the effector AvrRps4. Induction does not lead to hypersensitive cell death response (HR) but can induce electrolyte leakage, which often correlates with plant cell death. Activation of RRS1 and RPS4 without pathogens cannot activate mitogen-associated protein kinase cascades, but still activates up-regulation of defence genes, and therefore resistance against bacteria.