Adaptive evolution in single species bacterial biofilms
Adaptive evolution in single species bacterial biofilms
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DOI：
10.1111/j.1574-6968.2009.01526.x
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发表时间：
2009-04-01
影响因子：
2.1
通讯作者：
Finkel, Steven E.
中科院分区：
文献类型：
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作者：
Kraigsley, Alison M.;Finkel, Steven E.
关于细菌生物膜内细胞生长、死亡和进化的动力学知之甚少。在这里，我们展示了真实的时间内单物种生物膜进化的证据。从22天的生物膜收获的大肠杆菌比在生物膜中孵育较短时间的细胞表现出竞争优势。这种优势表现为在存在预先存在的生物膜的情况下，老化细胞胜过年轻细胞的能力，即使来自较老生物膜的细胞在新鲜的未被占据的表面上不表现出增加的形成初始生物膜的能力。这种现象类似于在竞争条件下孵育时，通过非稳态生长的细胞表现出的稳定期或GASP表型中的生长优势。生物膜中的细菌表现出快速遗传变化的能力对我们理解生物膜在各种自然和人造环境中的适应能力具有重要意义。
Little is known about the dynamics of cellular growth, death, and evolution within bacterial biofilms. Here we show evidence of evolution within single-species biofilms in real time. Escherichia coli harvested from 22-day-old biofilms express a competitive advantage over cells incubated in biofilms for shorter periods of time. This advantage is manifested as the ability of aged cells to outcompete younger cells in the presence of a pre-existing biofilm, even though cells from older biofilms do not express an increased ability to form initial biofilms on a fresh, unoccupied surface. This phenomenon is similar to the growth advantage in stationary phase, or GASP, phenotype exhibited by planktonically grown cells when incubated under competitive conditions. The ability of bacteria in biofilms to show rapid heritable change has implications for our understanding of the adaptive abilities of biofilms in a wide variety of natural and man-made environments.