Comparison of mouse brown and white adipose‑derived stem cell differentiation into pacemaker‑like cells induced by TBX18 transduction.
Comparison of mouse brown and white adipose‑derived stem cell differentiation into pacemaker‑like cells induced by TBX18 transduction.
复制标题
DOI：
10.3892/mmr.2018.8792
复制
发表时间：
2018-05
影响因子：
3.4
通讯作者：
Yang XQ
中科院分区：
文献类型：
--
作者：
Sun AJ;Qiao L;Huang C;Zhang X;Li YQ;Yang XQ
本研究旨在比较T-box（TBX）18转导后棕色脂肪源性干细胞（BASC）和白色脂肪源性干细胞（WASC）向起搏样细胞的分化。用腺病毒-TBX 18体外转染小鼠BASCs和WASCs，诱导其向起搏样细胞分化。在转导后48 h，通过荧光显微镜测定转导率，并通过透射电镜观察细胞超微结构的变化。采用逆转录-定量聚合酶链反应、免疫荧光染色和western blot检测起搏细胞相关标志物TBX 18、TBX 3、肌节α-辅肌动蛋白（Sr）和超极化激活的环核苷酸门控通道4（HCN 4）的mRNA和蛋白表达。结果表明，BASCs和WASCs之间的转导率没有观察到显著差异。BASCs的超微结构比WASCs复杂，表明BASCs可能具有更好的分化为起搏样细胞的结构基础。TBX 18、TBX 3、Sr和HCN 4在分化干细胞中的mRNA和蛋白表达与各自的对照组相比显著增加。此外，与TBX 18-WASC相比，TBX 18-BASC中的表达水平显著更高。结论：TBX 18基因转导可促进BASCs和WASCs向心肌起搏样细胞分化，且BASCs的心肌起搏样细胞分化能力高于WASCs。因此，TBX 18基因可能作为一个有效的候选人，在细胞移植治疗疾病和未来的研究，心血管系统。
The present study aimed to compare brown adipose-derived stem cell (BASC) and white adipose-derived stem cell (WASC) differentiation into pacemaker-like cells following T-box (TBX)18 transduction. Mouse BASCs and WASCs were induced to differentiate into pacemaker-like cells by adenovirus-TBX18 transduction in vitro. The transduction rate was determined by fluorescence microscopy and cell ultrastructural changes were observed by transmission electron microscopy at 48 h post-transduction. The mRNA and protein expression of pacemaker cell-associated markers, including TBX18, TBX3, sarcomeric α-actinin (Sr) and hyperpolarization-activated cyclic nucleotide-gated channel 4 (HCN4), were detected by reverse transcription-quantitative polymerase chain reaction, immunofluorescence staining and western blot analysis. The results demonstrated that no significant difference was observed in the transduction rate between BASCs and WASCs. The ultrastructure of BASCs was observed to be more complex than that of WASCs, indicating that BASCs may possess a better structural foundation to differentiate into pacemaker-like cells. TBX18, TBX3, Sr and HCN4 mRNA and protein expression in differentiated stem cells was significantly increased compared with the respective control groups. Furthermore, the expression levels were significantly higher in TBX18-BASCs compared with TBX18-WASCs. In conclusion, TBX18 gene transduction may facilitate the differentiation of BASCs and WASCs into pacemaker-like myocardial cells, and BASCs may have a higher capacity than WASCs for this differentiation. TBX18 gene may therefore act as an efficient candidate in cell transplantation therapy for diseases and for future research into the cardiovascular system.
登录
查看更多内容
影响因子：
46.9
作者：
通讯作者：
--
HCN4 动态标记第一个心脏场和传导系统前体
影响因子：
20.1
作者：
Liang X;Wang G;Lin L;Lowe J;Zhang Q;Bu L;Chen Y;Chen J;Sun Y;Evans SM
通讯作者：
Evans SM
影响因子：
10.5
作者：
Hoogaars, Willem M. H.;Engel, Angela;Christoffels, Vincent M.
通讯作者：
Christoffels, Vincent M.
影响因子：
64.8
作者：
Wainger, BJ;DeGennaro, M;Tibbs, GR
通讯作者：
Tibbs, GR
影响因子：
29
作者：
Seale, Patrick;Kajimura, Shingo;Spiegelman, Bruce M.
通讯作者：
Spiegelman, Bruce M.