HUMAN VISUAL PIGMENTS - MICROSPECTROPHOTOMETRIC RESULTS FROM THE EYES OF 7 PERSONS
HUMAN VISUAL PIGMENTS - MICROSPECTROPHOTOMETRIC RESULTS FROM THE EYES OF 7 PERSONS
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DOI：
10.1098/rspb.1983.0091
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
MOLLON, JD
中科院分区：
文献类型：
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作者：
DARTNALL, HJA;BOWMAKER, JK;MOLLON, JD
材料取自7只因恶性肿瘤而切除的眼睛。取视网膜的中心凹和视网膜旁样本，并使用Liebman显微分光光度计对视杆细胞和视锥细胞的外段的吸收光谱进行横向测量。在以下波长处获得具有吸收峰的四种光谱：棒，496.3 ±-。2.3 nm（n = 39）;红色视锥细胞，558.4 ±. 5.2 nm（n = 58）;绿色视锥细胞，530.8 ±. 3.5 nm（n = 45）;蓝色视锥细胞，419.0 ±. 3.6 nm（n = 5）。对于棒，峰的分布是单峰的。对于红色和绿色视锥细胞，有证据表明双峰分布，亚群最大值为563.2 ±。3.1 nm（n = 27）和554.2 ±. 2.3对于红色，在533.7 ± nm（n = 31）处。2.1 nm（n = 23）和527.8 ±. 1.8 nm（n = 22）用于绿色。在患者1（561 nm）和患者4（553 nm）之间观察到红色视锥细胞的平均光谱位置存在显著差异。这两名患者被归类为正常的三色视者的所有临床测试的色觉，但有一个明显的差异，他们的相对敏感性长波领域。在两个方向和幅度，这种差异被证明是所需的显微光谱的结果。
The material was obtained from 7 eyes removed because of malignant growths. Foveal and parafoveal samples of the retinas were taken and transverse measurements were made of the absorbance spectra of the outer segments of the rods and cones, using a Liebman microspectrophotometer. Four kinds of spectra were obtained with absorbance peaks at the following wavelengths: rods, 496.3 .+-. 2.3 nm (n = 39); red cones, 558.4 .+-. 5.2 nm (n = 58); green cones, 530.8 .+-. 3.5 nm (n = 45); blue cones, 419.0 .+-. 3.6 nm (n = 5). The distribution of the peaks was unimodal for the rods. For the red and green cones; there was evidence for bimodal distributions with subpopulation maxima at 563.2 .+-. 3.1 nm (n = 27) and 554.2 .+-. 2.3 nm (n = 31) for the reds and at 533.7 .+-. 2.1 nm (n = 23) and 527.8 .+-. 1.8 nm (n = 22) for the greens. A substantial difference in mean spectral location of the red cones was observed between patient 1 (561 nm) and patient 4 (553 nm). Both patients were classified as normal trichromats by all clinical tests of color vision but there was a clear difference in their relative sensitivities to long-wave fields. In both direction and magnitude, this difference proved to be that required by the microspectrometric results.