T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody
T-lymphocyte heterogeneity in the rat: separation of functional subpopulations using a monoclonal antibody
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大鼠 T 淋巴细胞异质性：使用单克隆抗体分离功能亚群
DOI：
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发表时间：
1978
影响因子：
15.3
通讯作者：
C. Milstein
中科院分区：
文献类型：
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作者：
R. White;D. Mason;A. Williams;G. Galfrè;C. Milstein
W3/25抗体是由小鼠骨髓瘤细胞系与来自用大鼠胸腺细胞免疫的小鼠的脾细胞融合产生的杂交细胞的单克隆产物。已经得到纯克隆，并且已经分离出不含亲本骨髓瘤链的分离体。以前的研究表明，这种抗体识别大鼠胸导管淋巴细胞中的T细胞亚群。本文报道了在荧光激活细胞分选仪上分离大鼠胸导管淋巴细胞后，用W3/25抗体标记T细胞，测定T细胞的三种功能。两个职能活动似乎完全分离的这一程序。因此，辅助细胞活性的抗半抗原空斑形成细胞反应被限制在标记的人口，而同种异体抑制作用产生的亲本载体F1过继转移介导的细胞在未标记的部分。第三个功能，移植物抗宿主活性，几乎完全包含在标记的亚群。结论：单克隆抗体W3/25识别的抗原决定簇是T细胞功能亚群的分化标志。
W3/25 antibody is the monoclonal product of a hybrid cell resulting from the fusion of a mouse myeloma cell line with spleen cells from a mouse immunized with rat thymocytes. Pure clones have been derived, and segregants free of parental myeloma chains have been isolated. Previous studies have shown that this antibody recognizes a subpopulation of T cells among rat thoracic duct lymphocytes. In the work reported here, three T-cell functions were assayed after separating rat thoracic duct lymphocytes on the fluorescence-activated cell sorter on the basis of labeling with W3/25 antibody. Two of the functional activities appeared to be completely segregated by this procedure. Thus, helper cell activity for an anti-hapten plaque-forming cell response was confined to the labeled population, whereas the allogeneic suppressive effect produced in a parental vector F1 adoptive transfer was mediated by cells in the unlabeled fraction. The third function, graft-versus-host activity, was almost entirely contained within the labeled subpopulation. It is concluded that the antigenic determinant recognized by the monoclonal antibody W3/25 is a differentiation marker for T-cell functional subpopulations.