Experimental model of tooth movement by orthodontic force in mice and its application to tumor necrosis factor receptor-deficient mice
Experimental model of tooth movement by orthodontic force in mice and its application to tumor necrosis factor receptor-deficient mice
复制标题
DOI：
10.1007/s00774-005-0641-4
复制
发表时间：
2006-01-01
影响因子：
3.3
通讯作者：
Yamaguchi, A
中科院分区：
文献类型：
--
作者：
Yoshimatsu, M;Shibata, Y;Yamaguchi, A
正畸牙齿移动是通过机械加载来实现的；然而，由于缺乏合适的实验模型，这一过程所涉及的生物学机制尚不清楚。在本研究中，我们使用插入上切牙和上第一磨牙之间的镍钛封闭螺旋弹簧建立了小鼠正畸牙齿移动模型。组织学检查表明，正畸力使第一上磨牙近中移动，牙齿移动过程中牙周组织没有坏死。压力侧TRAP阳性破骨细胞的数量以时间依赖性方式显着增加。基于实时定量的逆转录聚合酶链反应分析表明组织蛋白酶 K 的 mRNA 水平增加。免疫组织化学染色显示正畸牙齿移动期间第一磨牙压力侧牙周组织中肿瘤坏死因子-α (TNF α) 的表达。当将该牙齿移动系统应用于TNF 1型受体缺陷小鼠和TNF 2型受体缺陷小鼠时，在TNF 2型受体缺陷小鼠中观察到的牙齿移动小于野生型小鼠和TNF 1型受体缺陷小鼠。在应用矫治器后第6天，与TNF 1型受体缺陷型小鼠相比，TNF 2型受体缺陷型小鼠压力侧TRAP阳性破骨细胞的数量显着减少。本研究表明 TNF α 信号在正畸牙齿移动中发挥着重要作用。
Orthodontic tooth movement is achieved by mechanical loading; however, the biological mechanism involved in this process is not clearly understood owing to the lack of a suitable experimental model. In the present study, we established an orthodontic tooth movement model in mice using a Ni-Ti closed coil spring that was inserted between the upper incisors and the upper first molar. Histological examination demonstrated that the orthodontic force moved the first upper molar mesially without necrosis of the periodontium during tooth movement. The number of TRAP-positive osteoclasts on the pressure side significantly increased in a time-dependent manner. Quantitative real time-based reverse transcription-polymerase chain reaction analysis demonstrated increased levels of mRNA for cathepsin K. Immunohistochemical staining revealed the expression of tumor necrosis factor-alpha (TNF alpha) in periodontium on the pressure side of the first molar during orthodontic tooth movement. When this tooth movement system was applied to TNF type 1 receptor-deficient mice and TNF type 2 receptor-deficient mice, tooth movement observed in TNF type 2 receptor-deficient mice was smaller than that in the wild-type mice and TNF type 1 receptor-deficient mice. The number of TRAP-positive osteoclasts on the pressure side was significantly small in TNF type 2 receptor-deficient mice compared with that in TNF type 1 receptor-deficient mice on day 6 after application of the appliance. The present study indicates that TNF alpha signaling plays some important roles in orthodontic tooth movement.