Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks
Purification and Partial Characterization of Bacillus subtilis Flagellar Hooks
复制标题
枯草芽孢杆菌鞭毛钩的纯化和部分表征
作者：
K. Dimmitt;M. Simon
描述了一种制备细菌鞭毛钩结构的方法。该方法包括从产生热不稳定鞭毛丝的突变体中分离完整的鞭毛，并对它们进行热处理以优先解体鞭毛。所得的钩制剂可以通过速度和等密度离心分离和纯化。纯化的钩子沉淀物的相对S值为77。在十二烷基硫酸钠中进行丙烯酰胺凝胶电泳时，它们显示一条主要蛋白带和一些次要蛋白带。纯化的Hook可用于免疫家兔，所得抗血清是Hook特异性的。这些结果支持了这样的观点，即钩子是由一种不同于鞭毛蛋白的蛋白质组成的。
A method for preparing bacterial flagellar hook structures is described. The method involves isolating intact flagella from a mutant which makes thermally labile flagellar filaments and heat-treating them to disaggregate the filament preferentially. The resulting hook preparation can be separated and purified by velocity and isopycnic centrifugation. The purified hooks sediment at a relative S value of 77. On acrylamide gel electrophoresis in sodium dodecyl sulfate, they show one major and a number of minor protein bands. The purified hooks can be used to immunize rabbits, and the resulting antiserum is hook-specific. These results support the notion that hooks are composed of a protein that differs from flagellin.