Electrophysiological Recordings from Identified Cell Types in the Olfactory Cortex of Awake Mice.
Electrophysiological Recordings from Identified Cell Types in the Olfactory Cortex of Awake Mice.
复制标题
清醒小鼠嗅觉皮层中已识别细胞类型的电生理记录。
DOI：
10.1007/978-1-0716-3425-7_16
复制
发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Franks,KevinM
中科院分区：
文献类型：
--
作者：
Bolding,KevinA;Franks,KevinM
神经回路由无数不同类型的细胞组成，每种细胞都有特定的内在特性和突触连接模式，它们将神经输入转化并将信息传递给下游目标。了解气味刺激的不同特征是如何被编码并传递到适当的目标，将需要选择性地识别和操纵这些不同的电路元件。在这里，我们描述了在清醒、头部固定的小鼠的嗅觉（梨状）皮层中获得气味诱发活动的密集的细胞外电生理记录的方法，以及在该电路中识别遗传定义的细胞类型的光遗传学工具和程序。
Neural circuits consist of a myriad of distinct cell types, each with specific intrinsic properties and patterns of synaptic connectivity, which transform neural input and convey this information to downstream targets. Understanding how different features of an odor stimulus are encoded and relayed to their appropriate targets will require selective identification and manipulation of these different elements of the circuit. Here, we describe methods to obtain dense, extracellular electrophysiological recordings of odor-evoked activity in olfactory (piriform) cortex of awake, head-fixed mice, and optogenetic tools and procedures to identify genetically defined cell types within this circuit.