Oxalyl thiolester concentrations decreased in lectin and phorbol ester-stimulated lymphocytes.
Oxalyl thiolester concentrations decreased in lectin and phorbol ester-stimulated lymphocytes.
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凝集素和佛波酯刺激的淋巴细胞中草酰硫醇酯浓度降低。
DOI：
10.1096/fasebj.3.12.2792647
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Hamilton,GA
中科院分区：
文献类型：
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作者：
Skorczynski,SS;Mastro,AM;Hamilton,GA
草酰硫醇酯（OTEs）是一类新发现的哺乳动物代谢产物，被认为具有控制动物代谢的功能，并可能作为某些激素的细胞内介质。以前的相关性表明，当细胞被刺激增殖时，OTE的浓度可能会降低，在我们的研究中发现情况确实如此。因此，当牛淋巴结淋巴细胞用伴刀豆球蛋白A（Con A）或植物血凝素（PHA）和佛波酯12-O-十四烷酰基-佛波醇-13-乙酸酯（TPA）的组合刺激时，淋巴细胞中OTE的浓度在3小时内降低约2倍。单独使用PHA或TPA时，OTE浓度的降低相当小。使用Con A作为刺激剂，OTE水平在1 h内降低，并保持低水平至少24 h。还注意到，从2岁动物中获得的淋巴细胞中未刺激的分离淋巴细胞中的OTE浓度显著低于从老年动物中获得的淋巴细胞。目前的研究结果，当与其他最近的结果一起考虑时，表明OTE可能是天然的细胞增殖抑制剂。Skorczynski，S. S.的; Mastro，A. M.;汉密尔顿，G。A.草酰硫酯浓度在凝集素和佛波醇酯刺激的淋巴细胞中降低。FASEB J.3：2415 - 2419; 1989。
Oxalyl thiolesters (OTEs) are a newly discovered class of mammalian metabolites that are believed to function in controlling animal metabolism and possibly serve as intracellular mediators for some hormones. Previous correlations had suggested that the concentrations of OTEs might be decreased when cells are stimulated to proliferate, and in our research that was found to be the case. Thus, when bovine lymph node lymphocytes are stimulated either with concanavalin A (Con A) or with a combination of phytohemagglutinin (PHA) and the phorbol ester 12‐O‐tetradecanoyl‐phorbol‐13‐acetate (TPA), the concentration of OTEs in the lymphocytes decreases within 3 h by a factor of approximately two. With either PHA or TPA alone, the decrease in OTE concentration is considerably smaller. With Con A as stimulant, the OTE levels decrease within 1 h and remain low for at least 24 h. It was also noted that the concentration of OTEs in unstimulated isolated lymphocytes is significantly lower in lymphocytes obtained from 2‐year‐old animals than in lymphocytes obtained from older animals. The results of the current investigation, when considered in conjunction with other recent results, suggest that OTEs may be natural cell proliferation inhibitors.—Skorczynski, S. S.; Mastro, A. M.; Hamilton, G. A. Oxalyl thiolester concentrations decreased in lectin and phorbol ester‐stimulated lymphocytes.FASEB J.3: 2415‐2419; 1989.