3 DIFFERENT PATTERNS OF HEPATITIS-C VIRUS-INFECTION IN CHIMPANZEES
3 DIFFERENT PATTERNS OF HEPATITIS-C VIRUS-INFECTION IN CHIMPANZEES
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DOI：
10.1002/hep.1840150423
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发表时间：
1992-04-01
期刊：
影响因子：
13.5
通讯作者：
PRINCE, AM
中科院分区：
文献类型：
--
作者：
ABE, K;INCHAUSPE, G;PRINCE, AM
通过巢式聚合酶链反应和酶联免疫吸附测定，对从实验性感染丙型肝炎病毒的八只黑猩猩获得的连续血清样本中测定了丙型肝炎病毒RNA和丙型肝炎病毒相关抗体（针对假定的衣壳蛋白的抗体和C-100抗体）之间的关系。 聚合酶链式反应数据显示出三种不同的模式：第一种（第 1 组）是急性消退性肝炎，短暂出现 HCV RNA（两例）。 第二组（第2组）为慢性肝炎，丙型肝炎病毒RNA持续呈阳性（4例），第三组（第3组）为慢性肝炎，丙型肝炎病毒RNA间歇性出现（2例）。 接种后 1 周，八只动物中的四只首次在血清中检测到丙型肝炎病毒 RNA。 尽管在所有受感染的黑猩猩中均检测到血清 HCV RNA，但其中两只仅针对假定的衣壳蛋白抗体呈阳性，而两只仅针对 C-100 抗原抗体呈阳性。 在八个病例中的四个中，针对推定衣壳蛋白的抗体比针对 C-100 抗原的抗体出现得更早，在谷氨酸丙酮酸转氨酶值上升之前或同时检测到，并且即使在恢复后也长时间保持阳性。 接种1年后，八只动物中有六只（75%）仍呈丙型肝炎病毒RNA阳性，这表明丙型肝炎病毒感染后发展为慢性携带者状态的风险很高。 此外，血清中的抗体滴度与丙型肝炎病毒感染滴度之间似乎没有良好的相关性。
The relationship between hepatitis C virus RNA and hepatitis C virus-associated antibodies (antibody against the putative capsid protein and C-100 antibody) was determined by nested polymerase chain reaction and enzyme-linked immunosorbent assay in serial serum samples obtained from eight chimpanzees experimentally infected with hepatitis C virus. Three different patterns emerged from the polymerase chain reaction data: the first (group 1) was acute resolving hepatitis with transient appearance of HCV RNA (two cases). The second (group 2) had chronic hepatitis with persistent hepatitis C virus RNA positivity (four cases) and the third (group 3) had chronic hepatitis with intermittent appearance of hepatitis C virus RNA (two cases). In four of eight animals, hepatitis C virus RNA was first detectable in serum 1 wk after inoculation. Although serum HCV RNA was detected in all infected chimpanzees, two were positive only for antibody against the putative capsid protein, whereas two were positive only for antibody to C-100 antigen. In four of eight cases, antibody against the putative capsid protein appeared earlier than did antibody to C-100 antigen, was detected just before or coincident with rising glutamate pyruvate transaminase values and remained positive for a long time even after recovery. Six of eight animals (75%) were still hepatitis C virus RNA positive 1 yr after inoculation, suggesting that the risk of development of the chronic carrier state is high in hepatitis C virus infection. Furthermore, there did not appear to be a good correlation between antibody titer in serum and hepatitis C virus infectivity titer.