DIFFERENTIAL REGULATION OF C3 GENE-EXPRESSION IN HUMAN ASTROGLIOMA CELLS BY INTERFERON-GAMMA AND INTERLEUKIN-1-BETA
DIFFERENTIAL REGULATION OF C3 GENE-EXPRESSION IN HUMAN ASTROGLIOMA CELLS BY INTERFERON-GAMMA AND INTERLEUKIN-1-BETA
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DOI：
10.1016/0304-3940(95)11923-k
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发表时间：
1995-09-08
影响因子：
2.5
通讯作者：
JONES, JL
中科院分区：
文献类型：
--
作者：
BARNUM, SR;JONES, JL
在本报告中，我们研究了干扰素- γ (ifn - γ)和白细胞介素-1 β (IL-1 β)介导的补体第三组分C3表达的调节。白细胞介素-1 β诱导C3蛋白表达比ifn - γ快10倍。ifn - γ刺激C3表达需要从头蛋白合成，而环己亚胺和IL-1 β处理细胞可显著增加C3表达。放线菌素D抑制了ifn - γ和IL-1 β对C3基因的诱导，这表明这些细胞因子在转录水平上部分地增强了C3的表达。了解星形胶质细胞中补体基因表达的细胞因子介导调节对于确定这些分子在中枢神经系统炎症和自身免疫性疾病中的作用非常重要。
In this report, we examined interferon-gamma (IFN-gamma) and interleukin-1 beta IL-1 beta)-mediated regulation of the expression of C3, the third component of complement, in a human astroglioma cell line. Interleukin-1 beta induced C3 protein expression ten-fold more rapidly than IFN-gamma. De novo protein synthesis was required for IFN-gamma to stimulate C3 expression, while cycloheximide and IL-1 beta treatment of cells markedly increased C3 expression. Actinomycin D, inhibited C3 gene induction by IFN-gamma and IL-1 beta suggesting that these cytokines act, in part, at the transcriptional level to enhance C3 expression. Understanding cytokine-mediated regulation of complement gene expression in the astrocyte is important in defining the role of these molecules in CNS inflammation and autoimmune diseases.