Aggregates of ultrafine particles modulate lipid peroxidation and bacterial killing by alveolar macrophages
Aggregates of ultrafine particles modulate lipid peroxidation and bacterial killing by alveolar macrophages
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DOI：
10.1016/j.envres.2007.01.002
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发表时间：
2007-06-01
影响因子：
8.3
通讯作者：
Jarstrand, Connie
中科院分区：
文献类型：
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作者：
Lundborg, Margot;Bouhafs, Rabea;Jarstrand, Connie
我们假设超细碳和洗涤柴油颗粒的聚集体损害肺泡巨噬细胞（AM）杀死细菌的能力并增强AM肺表面活性物质的脂质过氧化（LPO）。将含有碳或洗涤的柴油颗粒的AM与肺炎链球菌（Streptococcus pneumoniae）、美国典型培养物保藏中心（ATCC）菌株或临床分离株孵育2小时。对存活细菌进行定量。将表面活性剂与碳或洗涤过的柴油负载的AM一起温育5小时，并测量LPO。金属浓度是10至100倍高，在洗柴油比碳颗粒。有一个剂量依赖性的增加，细菌存活与碳负载的巨噬细胞，但不是与洗涤柴油负载AM。临床分离株与碳负载巨噬细胞的存活率高于ATCC菌株。与对照组相比，表面活性剂LPO随着洗涤的装载柴油的巨噬细胞（95%）和装载碳的巨噬细胞（55%）而增加。额外的氧代谢产物维持了AM的杀菌活性，而相应的活性在载碳AM中降低。AM功能的改变可以解释与空气污染有关的健康问题。(C)2007年爱思唯尔公司All rights reserved.
We hypothesized that aggregates of ultrafine carbon and washed diesel particles impair the ability of alveolar macrophages (AM) to kill bacteria and enhance the AM lipid peroxidation (LPO) of lung surfactant.Rat AM were exposed, 5 h, to particles 20 mu g/ml. The AM, containing carbon or washed diesel particles, were incubated 2 h, with Streptococcus pneumoniae, an American Type Culture Collection (ATCC) strain or clinical isolates. Surviving bacteria were quantified. Surfactant was incubated, 5 h, with carbon or washed diesel loaded AM and LPO was measured.The particle load was approximately mu g/10(6) AM, representing accepted exposure to ambient particles in Europe. Metal concentrations were 10 to 100 fold higher in washed diesel-than in carbon particles. There was a dose dependent increase in bacterial survival with carbon-loaded macrophages, but not with washed diesel-loaded AM. Clinical isolates had a higher survival rate with carbon-loaded macrophages than the ATCC strain. Surfactant LPO was increased with washed diesel-loaded macrophages (95%) and with carbon-loaded macrophages (55%) compared to controls.High LPO caused by washed diesel-loaded AM reflects their increased oxidative metabolism, probably caused by particle metals. The additional oxygen metabolites maintained bactericidal activity of AM, while corresponding activity was decreased in carbon-loaded AM. Altered functions of AM may explain health problems related to air pollution. (C) 2007 Elsevier Inc. All rights reserved.