Adult Epicardial Fat Exhibits Beige Features
Adult Epicardial Fat Exhibits Beige Features
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DOI：
10.1210/jc.2013-1265
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发表时间：
2013-09-01
影响因子：
5.8
通讯作者：
Symonds, Michael E.
中科院分区：
文献类型：
--
作者：
Sacks, Harold S.;Fain, John N.;Symonds, Michael E.
背景：人类心外膜脂肪以前被指定为棕色样脂肪。基于其基因表达谱，人类锁骨上脂肪库被定义为米色与经典棕色共存。目的：本研究的目的是建立人类心外膜和内脏心旁脂肪与皮下脂肪相比的基因表达谱和形态。设置：本研究在三级护理医院心脏中心进行。患者：心外膜、心旁内脏和皮下脂肪样本取自患有严重冠状动脉粥样硬化或心脏瓣膜病的中年患者。通过逆转录-定量PCR和线粒体解偶联蛋白-1（UCP-1）的相对丰度测定基因表达Western blotting。心外膜组织切片的患者进行了检查，通过光学显微镜，UCP-1免疫组化，和细胞morphometry.Main结果Measures：我们假设，心外膜脂肪具有混合表型与基因表达谱描述的米色细胞lineage.Results：免疫反应UCP-1是明确可测量的每个心外膜样本分析，但在其他4个内脏和SC仓库检测不到。心外膜脂肪表现出UCP-1、PRDM 16、PGC-1 α、PPAR γ和米色脂肪细胞特异性标志物CD 137的基因的高表达，这些基因也在内脏心旁脂肪中表达，但在胸骨、上腹部和下肢皮下脂肪中仅弱表达。心外膜脂肪的组织学表现为小的单室脂肪细胞无UCP-1免疫staining.Conclusion：UCP-1是相对丰富的心外膜脂肪，这个仓库具有分子特征的那些在体外发现的米色谱系脂肪细胞。
Context: Human epicardial fat has been designated previously as brown-like fat. The supraclavicular fat depot in man has been defined as beige coexistent with classical brown based on its gene expression profile.Objective: The aim of the study was to establish the gene expression profile and morphology of human epicardial and visceral paracardial fat compared with sc fat.Setting: The study was conducted at a tertiary care hospital cardiac center.Patients: Epicardial, visceral paracardial, and sc fat samples had been taken from middle-aged patients with severe coronary atherosclerosis or valvular heart disease.Interventions: Gene expression was determined by reverse transcription-quantitative PCR and relative abundance of the mitochondrial uncoupling protein-1 (UCP-1) by Western blotting. Epicardial tissue sections from patients were examined by light microscopy, UCP-1 immunohistochemistry, and cell morphometry.Main Outcome Measures: We hypothesized that epicardial fat has a mixed phenotype with a gene expression profile similar to that described for beige cell lineage.Results: Immunoreactive UCP-1 was clearly measurable in each epicardial sample analyzed but was undetectable in each of the 4 other visceral and sc depots. Epicardial fat exhibited high expression of genes for UCP-1, PRDM16, PGC-1 alpha, PPAR gamma, and the beige adipocyte-specific marker CD137, which were also expressed in visceral paracardial fat but only weakly in sternal, upper abdominal, and lower extremity sc fat. Histology of epicardial fat showed small unilocular adipocytes without UCP-1 immunostaining.Conclusion: UCP-1 is relatively abundant in epicardial fat, and this depot possesses molecular features characteristic of those found in vitro in beige lineage adipocytes.