TGase regulates salt stress tolerance through enhancing bound polyamines-mediated antioxidant enzymes activity in tomato
TGase regulates salt stress tolerance through enhancing bound polyamines-mediated antioxidant enzymes activity in tomato
复制标题
TGase 通过增强番茄中结合多胺介导的抗氧化酶活性来调节盐胁迫耐受性
DOI：
10.1016/j.envexpbot.2020.104191
复制
发表时间：
2020
影响因子：
5.7
通讯作者：
Guo Shirong
中科院分区：
文献类型：
--
作者：
Zhong Min;Song Ran;Wang Yu;Shu Sheng;Sun Jin;Guo Shirong
The accumulation of free polyamines (PAs) plays critical role in plants under abiotic stress conditions, while protective traits of bound PAs receiving much less attention, our understanding of the regulation of free PAs converted to bound PAs is limited. In this study, we demonstrated that the PA mediator transglutaminase (TGase) play an important role in improving tomato tolerance to salinity stress, in part because its critical role in inducing accumulation of bound PAs under salt stress.TGaseexpression was induced upon salt stress. Exogenous phenanthroline (O-phen, a TGase and bound PAs synthesis inhibitor) abolished the TGase-mediated salt tolerance with terminating bound PAs accumulation inTGaseOE plants. O-phen also had a negatively altered on Na+/K+uptake or homeostasis under salt stress in leaves ofTGaseOE plants. Instead, the overexpression ofTGaseeffectively alleviated salt-induced oxidative damage and reduced reactive oxygen species (ROS) over-production, but it was significantly aggravated by O-phen in theTGaseOE plants. Although the ROS generating enzymes (PA oxidase, diamine oxidase and NADPH oxidase) was not affected by application of O-phen, the antioxidant enzyme (superoxide dismutase, catalase, ascorbate peroxidase and monodehydroascorbate reductase) activity was significantly decreased by exogenous O-phen in plants. Furthermore, further analysis showed that the antioxidant enzyme activity positively and closely correlated with the level of bound PAs inTGaseOE plant. Together, these results uncovered a probably function mechanism of TGase-mediated antioxidant enzyme activity to reduced salt-induced oxidative damage, which is partially depended on bound PAs accumulation.
