Fluorescent sp3 Defect-Tailored Carbon Nanotubes Enable NIR-II Single Particle Imaging in Live Brain Slices at Ultra-Low Excitation Doses
Fluorescent sp3 Defect-Tailored Carbon Nanotubes Enable NIR-II Single Particle Imaging in Live Brain Slices at Ultra-Low Excitation Doses
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DOI：
10.1038/s41598-020-62201-w
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发表时间：
2020-03-24
影响因子：
4.6
通讯作者：
Cognet, Laurent
中科院分区：
文献类型：
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作者：
Mandal, Amit Kumar;Wu, Xiaojian;Cognet, Laurent
在第二近红外窗口（NIR-II，类似于1000-1350 nm）中的细胞和组织成像对于体内研究是有利的，因为在这些波长下生物成分的低消光。然而，由于缺乏合适的生物探针，在NIR-II窗口中尚未实现单分子灵敏度的深部组织成像。单壁碳纳米管已经成为有前途的近红外发光分子生物探针;然而，它们低效的光致发光（量子产率类似于1%）驱动了对相当大的激发剂量（类似于1-10 kW/cm（2））的需求，这些激发剂量从NIR-II区域显著蓝移（
Cellular and tissue imaging in the second near-infrared window (NIR-II, similar to 1000-1350 nm) is advantageous for in vivo studies because of low light extinction by biological constituents at these wavelengths. However, deep tissue imaging at the single molecule sensitivity has not been achieved in the NIR-II window due to lack of suitable bio-probes. Single-walled carbon nanotubes have emerged as promising near-infrared luminescent molecular bio-probes; yet, their inefficient photoluminescence (quantum yield similar to 1%) drives requirements for sizeable excitation doses (similar to 1-10 kW/cm(2)) that are significantly blue-shifted from the NIR-II region (