Stromal stem cells and platelet-rich plasma improve bone allograft integration
Stromal stem cells and platelet-rich plasma improve bone allograft integration
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DOI：
10.1097/01.blo.0000165736.87628.12
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发表时间：
2005-06-01
影响因子：
4.2
通讯作者：
Donati, D
中科院分区：
文献类型：
--
作者：
Lucarelli, E;Fini, M;Donati, D
早期血管侵袭是同种异体骨移植合并的关键因素。它可以减少与骨整合缓慢和不完全相关的并发症。与富含血小板的血浆相关的骨髓源性基质干细胞是有效的血管生成诱导剂，已被证明可以释放血管内皮生长因子。我们的目标是测试基质干细胞和富含血小板的血浆的组合是否能够在 4 个月时处死的大型动物模型中增加大规模同种异体移植物整合。 10只羊的跖骨中骨干出现严重缺损；研究组接受同种异体移植物加基质干细胞、富含血小板的血浆和胶原蛋白（六只动物），对照组仅接受同种异体移植物（四只动物）。通过放射线照片、机械测试和组织形态分析（包括新血管形成）进行研究。结果显示，研究组的同种异体移植物中有大量新骨形成。在研究组中，骨形成与更好的血管侵袭和移植物重塑相关。这些结果证实了基质干细胞和富含血小板的血浆在骨修复中发挥的关键作用。需要进一步的研究来更好地确定基质干细胞单独植入时所发挥的作用。
Early vascular invasion is a key factor in bone allograft incorporation. It may reduce the complications related to slow and incomplete bone integration. Bone-marrow-derived stromal stem cells associated with platelet-rich plasma are potent angiogenic inducers proven to release vascular endothelial growth factor. Our goal was to test whether the combination of stromal stem cells and platelet-rich plasma is able to increase massive allograft integration in a large animal model with sacrifice at 4 months. A critical defect was made in the mid-diaphysis of the metatarsal bone of 10 sheep; the study group received an allograft plus stromal stem cells, platelet-rich plasma, and collagen (six animals) and the control group received only the allograft (four animals). Investigation was done with radiographs, mechanical tests and histomorphometric analysis, including new vascularization. Results showed substantial new bone formation in the allograft of the study group. Bone formation is correlated with better vascular invasion and remodeling of the graft in the study group. These results confirm the key role played by stromal stem cells and platelet-rich plasma in bone repair. Further studies are needed to better define the role stromal stem cells play when implanted alone.