Adrenocorticotropin induces calcium oscillations in adrenal fasciculata cells: single cell imaging.
Adrenocorticotropin induces calcium oscillations in adrenal fasciculata cells: single cell imaging.
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促肾上腺皮质激素诱导肾上腺束状细胞中的钙振荡：单细胞成像。
DOI：
10.1006/bbrc.1996.0538
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发表时间：
1996
影响因子：
3.1
通讯作者：
S. Kawato
中科院分区：
文献类型：
--
作者：
T. Kimoto;Yoshihiro Ohta;S. Kawato
通过荧光显微镜成像，我们已经证明，在生理浓度为0.1-100 pM的促肾上腺皮质激素（ACTH）刺激下，钙绿-1负载的牛肾上腺束状细胞中发生Ca2+信号传导。我们观察到三种Ca2+信号模式，即Ca2+振荡（33%），Ca2+浓度阶梯式增加（10%），Ca2+振荡叠加在Ca2+阶梯式增加（57%）上。细胞内Ca2+浓度的振荡频率约为0.04 Hz。当ACTH刺激的Ca2+信号被EGTA抑制时，皮质激素的产生被显著抑制。结果表明，Ca2+信号可能是acth诱导的束状带细胞合成类固醇激素的第二信使。
With fluorescence microscopic imaging, we have demonstrated that the Ca2+ signaling occurred in individual Calcium Green-1 loaded bovine adrenal fasciculata cells upon stimulation with adrenocorticotropin (ACTH) at physiological concentration of 0.1-100 pM. We observed three patterns of Ca2+ signaling which were Ca2+ oscillations (33%), step-like increase in Ca2+ concentration (10%), and Ca2+ oscillations superimposed on step-like increase in Ca2+ (57%). The oscillation in intracellular Ca2+ concentration occurred with a frequency around 0.04 Hz. When Ca2+ signaling upon ACTH stimulation was inhibited by the treatment with EGTA, the corticoid production was considerably suppressed. The results suggest that the Ca2+ signaling is a probable candidate of the second messenger for ACTH-induced steroid hormone synthesis in zona fasciculata cells.