Limited compatibility between the RNA polymerase components of influenza virus type A and B
Limited compatibility between the RNA polymerase components of influenza virus type A and B
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DOI：
10.1016/j.virusres.2008.03.010
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发表时间：
2008-07-01
期刊：
影响因子：
5
通讯作者：
Horimoto, Taisuke
中科院分区：
文献类型：
--
作者：
Iwatsuki-Horimoto, Kiyoko;Hatta, Yasuko;Horimoto, Taisuke
虽然A型和B型流感病毒在人群中共同传播，但在自然界中尚未检测到这两种病毒之间的相互作用。对此的一种解释可能是A型和B型病毒之间RNA转录和复制的功能不相容性。为了验证这种可能性，我们构建了A/B型嵌合体聚合酶机制，其中包含来自两种病毒类型中的每一种的PB 2、PB 1、PA和核蛋白，并在报告分析中评估了它们与A型启动子的聚合活性。含有同源组分的B型聚合酶机器与A型启动子一起起作用，尽管在不同程度上取决于启动子序列下游区域所来源的区段，表明A型启动子和B型聚合酶机器之间的功能相容性。然而，所有的A/B镶嵌聚合酶机制，除了含有PA从A型和其他从B型病毒株，不与A型启动子的功能，表明有限的兼容性之间的聚合酶组分的两种类型。总之，这些数据表明，RNA转录和复制的聚合酶机制的组件之间的不相容性是不负责缺乏异型reflectants。(c)2008 Elsevier B. V.保留所有权利。
Reassortants between type A and B influenza viruses have not been detected in nature, although both viruses co-circulate in human populations. One explanation for this may be functional incompatibility of RNA transcription and replication between type A and B viruses. To test this possibility, we constructed type A/B mosaic polymerase machinery, containing PB2, PB1, PA and nucleoprotein from each of the two virus types, and assessed their polymeraise activities with a type A promoter in a reporter assay. Type B polymerase machinery containing homologous components was functional with the type A promoter albeit to various extents depending on the segments from which the regions downstream of the promoter sequence were derived, indicating functional compatibility between the type A promoter and B polymerase machinery. However, all of the A/B mosaic polymerase machinery, except that containing PA from a type A and the others from a type B virus strain, did not function with the type A promoter, indicating limited compatibility among polymerase components of both types. Taken together, these data suggest that incompatibility among components of the polymerase machinery for RNA transcription and replication alone is not responsible for the lack of heterotypic reassortants. (c) 2008 Elsevier B.V. All rights reserved.