LINE-1 hypomethylation in spermatozoa is associated with Bisphenol A exposure
LINE-1 hypomethylation in spermatozoa is associated with Bisphenol A exposure
复制标题
精子中的 LINE-1 低甲基化与双酚 A 暴露有关
DOI：
10.1111/j.2047-2927.2013.00166.x
复制
发表时间：
2014-01-01
期刊：
影响因子：
4.5
通讯作者：
Li, D. -K.
中科院分区：
文献类型：
--
作者：
Miao, M.;Zhou, X.;Li, D. -K.
双酚A（BPA）是一种内分泌干扰物，对人类有潜在的有害影响。然而，调节BPA影响的表观遗传机制仍不清楚。长散布核苷酸元件（LINE-1）的甲基化是全基因组甲基化状态的标志。本研究旨在检查BPA暴露是否与男性LINE-1甲基化变化相关。对湖南省某工厂149名男性工人进行了调查，其中77名为BPA接触组，72名为非BPA接触组。从BPA暴露组中采集轮班前和轮班后的尿液样本，从对照组中采集现场尿液样本。对尿液样本进行BPA评估。此外，从两组中收集血液和精液样品用于LINE-1甲基化分析。在校正年龄、教育、吸烟习惯和饮酒的多变量分析中，BPA暴露工人的精子LINE-1甲基化水平显著降低（p
Bisphenol A (BPA) is an endocrine disruptor with potentially harmful effects on humans. However, epigenetic mechanisms that modulate the effects of BPA remain unclear. Methylation of long interspersed nucleotide elements (LINE-1) is a marker of genome-wide methylation status. This study aims to examine whether BPA exposure was associated with LINE-1 methylation changes in men. Male factory workers in Hunan, China (N=149) were studied, 77 with BPA exposure in workplace (BPA-exposed group) and 72 without BPA exposure in workplace (control group). Pre-shift and post-shift urine samples were collected from the BPA-exposed group and spot urine samples were collected from the control group. Urine samples were assessed for BPA. In addition, blood and semen samples were collected from both groups for LINE-1 methylation analysis. In multivariate analysis adjusted for age, education, smoking habits and alcohol consumption, sperm LINE-1 methylation level was significantly lower in BPA exposed workers (p