Protective effects of a mixture of antioxidant vitamins and minerals on the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster
Protective effects of a mixture of antioxidant vitamins and minerals on the genotoxicity of doxorubicin in somatic cells of Drosophila melanogaster
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DOI：
10.1002/em.20160
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Nepomuceno, JC
中科院分区：
文献类型：
--
作者：
Costa, WF;Nepomuceno, JC
抗氧化维生素能够使细胞生化过程中产生的高生物活性分子失活，例如自由基。阿霉素 (DXR) 是一种癌症化疗药物，会产生自由基作为副产物。在本研究中，采用黑腹果蝇体细胞翼斑试验来评估维生素（维生素 C、E 和 P-胡萝卜素）和矿物质（铜、硒和锌）混合物（商业名称为 Vitergan(R) Zinc Plus）对果蝇标准和高生物活性杂交中 DXR 遗传毒性的影响。 12.5、25和50mg/ml的维生素/矿物质混合物本身对于两个杂交的反式杂合后代是无基因毒性的，而该混合物使0.125mg/ml DXR在两个杂交的反式杂合后代中产生的基因毒性显着降低。当幼虫接受多种维生素/矿物质（MV）混合物的预处理或联合处理时，观察到了保护作用。结果表明，在这些实验条件下，MV混合物不具有遗传毒性；然而，它可以防止化疗自由基发生器 DXR 的基因毒性作用。
Antioxidant vitamins are able to deactivate highly bioactive molecules, such as free radicals, that are generated during cellular biochemical processes. Doxorubicin (DXR) is a cancer chemotherapeutic agent that generates free radicals as a byproduct. In the present study, the Drosophila melanogaster somatic wing spot test was used to evaluate the effects of a mixture of vitamins (Vitamins C, E, and P-carotene) and minerals (copper, selenium, and zinc), commercially known as Vitergan(R) Zinc Plus, on the genotoxicity of DXR in standard and high-bioactivation crosses of flies. 12.5, 25, and 50 mg/ml of the vitamin/mineral mixture by itself was nongenotoxic in the trans-heterozygous descendants of both crosses, while the mixture produced a significant reduction in the genotoxicity produced by 0.125 mg/ml DXR in the trans-heterozygous descendants of both crosses. The protective effect was observed when the larvae received either pre- or cotreatments of the multivitamin/mineral (MV) mixture. The results indicate that, under these experimental conditions, the MV mixture is not genotoxic; however, it protects against the genotoxic effects of the chemotherapeutic free-radical generator DXR.