Three-Dimensional Highly Sensitive Diffusion Reflection-Based Imaging Method for the in Vivo Localization of Atherosclerosis Plaques Following Gold Nanorods Accumulation.
Three-Dimensional Highly Sensitive Diffusion Reflection-Based Imaging Method for the in Vivo Localization of Atherosclerosis Plaques Following Gold Nanorods Accumulation.
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DOI：
10.1021/acsomega.8b00750
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发表时间：
2018-06-30
期刊：
影响因子：
4.1
通讯作者：
Fixler D
中科院分区：
文献类型：
--
作者：
Ankri R;Chakraborty R;Motiei M;Fixler D
在这项工作中，我们提出了一种新颖、简单且高精度的三维（3D）扩散反射（DR）成像系统和方法，用于检测组织内金纳米棒（GNR）的积累位点。 GNR 被广泛用于各种疾病的诊断目的，主要是因为它们能够很好地吸收可见光，这使得它们在各种成像和治疗诊断方法中成为出色的造影剂。最近，这些 GNR 独特的吸收特性已用于基于 DR 强度的测量，这表明了一种新型诊断工具 DR-GNR。在本文中，我们展示了一种新的 DR 测量系统和方法，基于从组织中进行的径向收集。这些径向测量实现了 DR-GNR 的独特 3D 呈现，引入了半径尺寸 ρ、角度尺寸 θ 和反射强度尺寸 Γ。基于扩散模型，可以将样品的光学特性与其反射率关联起来，呈现出独特的径向图。该图引入了每个测量角度的 DR 曲线的斜率，该斜率与组织的光学特性和组织内的 GNR 浓度线性相关，从而实现了样本中 GNR 的精确径向定位。我们展示了组织样体模中巨噬细胞积累的检测，以及高脂血症小鼠体内不稳定斑块的定位。这种高精度、强大的技术为实时检测方法铺平了道路，可以成功地集成到快速增长的个性化医疗领域。
In this work, we present a novel, simple, and highly accurate three-dimensional (3D) diffusion reflection (DR) imaging system and method for the detection of accumulation sites of gold nanorods (GNRs) within the tissue. GNRs are intensively used for diagnosis purposes of varied diseases, mainly because of their ability to well absorb visible light, which introduces them as terrific contrast agents in various imaging and theranostics methods. Lately, these GNRs unique absorption properties have served in DR intensity-based measurements, suggesting a novel diagnostic tool, DR-GNRs. In this paper, we show a new measurement system and method for DR, based on its radial collection from the tissue. These radial measurements enabled a unique 3D presentation of the DR-GNR, introducing the dimensions ρ for the radius, θ for the angle, and Γ for the reflected intensity. On the basis of the diffusion model, which enables to correlate between the sample’s optical properties and its reflectance, a unique, radial map is presented. This map introduces the slopes of the DR curves in each measured angle, which are linearly correlated with the tissue’s optical properties and with the GNRs concentrations within the tissue, thus enables the exact radial localization of the GNRs in the sample. We show the detection of macrophage accumulation in tissue-like phantoms, as well as the localization of unstable plaques in hyperlipidemic mice, in vivo. This highly accurate, powerful technology paves the way toward a real-time detection method that can be successfully integrated in the rapid increasing field of personalized medicine.