Protection studies with recombinant excretory/secretory proteins of Haemonchus contortus
Protection studies with recombinant excretory/secretory proteins of Haemonchus contortus
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DOI：
10.1046/j.1365-3024.2002.00454.x
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发表时间：
2002-04
影响因子：
2.2
通讯作者：
L. Vervelde;M. V. van Leeuwen;M. Kruidenier;F. Kooyman;J. Huntley;I. van Die;A. Cornelissen
中科院分区：
文献类型：
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作者：
L. Vervelde;M. V. van Leeuwen;M. Kruidenier;F. Kooyman;J. Huntley;I. van Die;A. Cornelissen
研究了捻转血矛线虫的两种重组蛋白在成年绵羊和幼羔羊中的效力。将这些15和24 kDa排泄/分泌蛋白组合给予，补充或不补充富含聚糖的昆虫细胞提取物。在9月龄绵羊（试验1）中，接种rec 15/24后，粪便虫卵排出量和蠕虫负荷分别减少49%和55%，接种rec 15/24和聚糖提取物后分别减少46%和65%。使用上述重组蛋白加富含聚糖的提取物（试验2），未发现幼龄羔羊的产蛋量或蠕虫数量减少。当重复试验1时（试验3），保护不能再现，可能是由于重组蛋白批次的差异。在所有绵羊中，不受年龄影响，均存在rec 15/24特异性免疫球蛋白（IG）G1和伊加滴度，但9月龄受保护绵羊的伊加滴度显著高于羔羊。添加聚糖导致攻毒后9月龄绵羊的rec 15/24特异性IgG 1和伊加较低。这并不影响保护的程度。在用补充有聚糖的rec 15/24免疫的保护绵羊中，发现伊加与蠕虫数量之间存在显著的负相关。总IgE和rec 15/24特异性IgE滴度较低。嗜酸性粒细胞，肥大细胞，羊肥大细胞蛋白酶（SMCP）+细胞和伊加+细胞的数量没有不同的保护和未保护的羊，但羔羊有显着较少的肥大细胞独立于他们的免疫。
The efficacy of two recombinant proteins of Haemonchus contortus was studied in both adult sheep and young lambs. These 15 and 24 kDa excretory/secretory proteins were given combined, either supplemented or not with a glycan‐rich insect cell extract. In 9‐month‐old sheep (trial 1), faecal egg output and worm burden were reduced by 49% and 55%, respectively, after vaccination with rec15/24, and by 46% and 65% after vaccination with rec15/24 and glycan extract. No reduction in egg output or number of worms was found in young lambs using the above recombinant proteins plus glycan‐rich extract (trial 2). When trial 1 was repeated (trial 3), the protection could not be reproduced, possibly due to differences in batches of recombinant proteins. In all sheep, independent of their age, rec15/24‐specific immunoglobulin (Ig)G1 and IgA titres were present, but 9‐month‐old protected sheep had significantly higher IgA titres than the lambs. Addition of glycans resulted in lower rec15/24‐specific IgG1 and IgA in 9‐month‐old sheep after challenge. This did not affect the level of protection. A significant negative correlation was found between IgA and worm numbers in protected sheep immunized with rec15/24 supplemented with glycans. Total IgE and rec15/24 specific IgE titres were low. The number of eosinophils, mast cells, sheep mast cell protease (SMCP)+ cells and IgA+ cells did not differ between the protected and unprotected sheep, but the lambs had significantly fewer mast cells independent of their immunization.