Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering
Hyaluronic acid modified biodegradable scaffolds for cartilage tissue engineering
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DOI：
10.1016/j.biomaterials.2004.06.021
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发表时间：
2005-05-01
期刊：
影响因子：
14
通讯作者：
Park, TG
中科院分区：
文献类型：
--
作者：
Yoo, HS;Lee, EA;Park, TG
将透明质酸（hyaluronan，HA）固定在大孔生物可降解聚（D，L-乳酸-羟基乙酸）[PLGA]支架表面，以增强支架的附着、增殖。以及软骨细胞的分化，用于软骨组织工程。通过将PLGA与不同量的胺封端的PLGA-PEG二嵌段共聚物共混来制备PLGA支架。它们是通过气体发泡/盐浸法制造的。HA与预制支架上表面暴露的胺基化学缀合。表面暴露的游离胺基的量通过将胺反应性荧光染料缀合至PLGA共混物膜来定量测定。HA固定化的程度也通过测量水接触角来证实。当软骨细胞接种在HA修饰的PLGA支架内时，与未修饰的PLGA支架相比，观察到增强的细胞附着。此外，HA修饰的PLGA支架的糖胺聚糖和总胶原合成显著增加。RT-PCR结果和所得软骨组织的组织学检查显示，HA改性支架在诱导软骨组织形成方面表现出优异的11型胶原表达和组织形态学特征。(C)2004爱思唯尔有限公司保留所有权利。
Hyaluronic acid (hyaluronan, HA) was immobilized onto the surface of macroporous biodegradable poly(D,L-lactic acid-co-glycolic acid) [PLGA] scaffolds to enhance the attachment, proliferation. and differentiation of chondrocyles for cartilage tissue engineering. The PLGA scaffolds were prepared by blending PLGA with varying amounts of amine-terminated PLGA-PEG di-block copolymer. They were fabricated by a gas foaming/salt leaching method. HA was chemically conjugated to the surface-exposed amine groups on the pre-fabricated scaffolds. The amount of surface exposed free amine groups was quantitatively determined by conjugating an amine-reactive fluorescent dye to the PLGA blend films. The extent of HA immobilization was also confirmed by measuring water contact angles. When chondrocytes were seeded within HA modified PLGA scaffolds, enhanced cellular attachment was observed compared to unmodified PLGA scaffolds. Furthermore, glycosaminoglycan and total collagen synthesis increased substantially for HA modified PLGA scaffolds. RT-PCR result and histological examination of the resultant cartilage tissue revealed that HA modified scaffolds excelled in inducing cartilage tissue formation in terms of collagen type 11 expression and tissue morphological characteristics. (C) 2004 Elsevier Ltd. All rights reserved.