Altered expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder by 17β-estradiol
Altered expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder by 17β-estradiol
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DOI：
10.1186/1471-2490-13-44
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发表时间：
2013-09-16
期刊：
影响因子：
2
通讯作者：
Park, Kwangsung
中科院分区：
文献类型：
--
作者：
Kim, Sun-Ouck;Song, Seung Hee;Park, Kwangsung
背景资料：方法：将雌性SD大鼠随机分为对照组、双侧卵巢切除组（Ovx）和双侧卵巢切除后皮下注射17 β-雌二醇组（Ovx + Est），观察雌激素水平变化对膀胱平滑肌细胞Caveolin 2和Caveolin 3表达的影响。4周后进行尿流动力学检查，确定收缩间期和收缩压。采用Western blot和免疫组织化学方法检测Caveolin 2和Caveolin 3在大鼠膀胱平滑肌中的表达和细胞定位。在膀胱测压图中，Ovx组的收缩间隔（min）（3.1 +/- 1.5）显著低于对照组（5.6 +/- 1.2），但在雌激素治疗后增加（9.3 +/- 1.0）。相反，Ovx组的平均收缩压（mmHg）（26.2 +/- 2.3）高于对照组（21.9 +/- 3.1），雌激素治疗后下降（23.8 +/- 3.5）。Caveolin 2和Caveolin 3的表达定位于平滑肌细胞膜。卵巢切除后，Caveolin 2和Caveolin 3的蛋白表达均显著降低，17 β-雌二醇处理后，Caveolin 2和Caveolin 3的蛋白表达恢复到对照组水平。结论：激素改变可导致大鼠膀胱平滑肌Caveolin 2和Caveolin 3的表达发生显著变化。这些研究结果表明，这些分子可能有逼尿肌过度活动，发生与激素改变的功能作用。
Background: The purpose of this study was to investigate the effect of estrogen alteration on the expression of caveolin 2 and 3 in rat smooth muscle of urinary bladder.Methods: Female Sprague-Dawley rats were divided into three groups: control, bilateral ovariectomy (Ovx), and bilateral ovariectomy followed by subcutaneous injections of 17 beta-estradiol (Ovx + Est). After 4 weeks, urodynamic measurements were taken to ascertain the contraction interval and contraction pressure. The expression and cellular localization of caveolin 2 and 3 were determined by Western blot and immunohistochemistry in rat urinary bladder smooth muscle.Results: In cystometrograms, the contraction interval (min) was significantly lower in the Ovx group (3.1 +/- 1.5) than in the control group (5.6 +/- 1.2), but was increased after estrogen treatment (9.3 +/- 1.0). Conversely, the average contraction pressure (mmHg) was higher in the Ovx group (26.2 +/- 2.3) than in the control group (21.9 +/- 3.1), and was decreased after estrogen treatment (23.8 +/- 3.5). Caveolin 2 and 3 expression was localized in the cell membrane of the smooth muscle. The protein expression of both caveolin 2 and 3 was significantly lower after ovariectomy and was restored to the control levels after 17 beta-estradiol treatment.Conclusions: Hormonal alteration causes a significant change in the expression of caveolin 2 and 3 in smooth muscle of rat urinary bladder. These findings suggest that these molecules might have functional roles in the detrusor overactivity that occurs in association with hormonal alteration.