Selective loss of human leukocyte class I allospecificities and staining of melanoma cells by monoclonal antibodies recognizing monomorphic determinants of class I human leukocyte antigens.
Selective loss of human leukocyte class I allospecificities and staining of melanoma cells by monoclonal antibodies recognizing monomorphic determinants of class I human leukocyte antigens.
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发表时间：
1993-07
期刊：
影响因子：
11.2
通讯作者：
T. Kageshita;Zhigang Wang;L. Calorini;A. Yoshii;T. Kimura;T. Ono;S. Gattoni‐Celli;S. Ferrone
中科院分区：
文献类型：
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作者：
T. Kageshita;Zhigang Wang;L. Calorini;A. Yoshii;T. Kimura;T. Ono;S. Gattoni‐Celli;S. Ferrone
从手术切除的黑色素瘤病变的冷冻切片的免疫过氧化物酶染色显示，抗人类白细胞抗原（HLA）-A2，A28单克隆抗体（mAb）染色角质形成细胞，但不染色黑色素瘤细胞在21%的14个原发性和44%的9个转移性病变测试。HLA-A2和/或A28同种特异性的丧失不影响mAb识别HLA I类抗原单态决定簇的染色模式，染色黑色素瘤细胞的百分比和染色强度。这一发现不太可能反映免疫过氧化物酶技术的敏感性，因为细胞荧光分析检测到，在黑色素瘤细胞系和基因转移后获得HLA-A2抗原的自体转染子之间，单克隆抗体对HLA I类抗原单态决定簇的染色模式无显著差异。本研究的结果表明，黑色素瘤细胞的HLA I类抗原表达异常的频率高于文献中所描述的，因为选择性损失的HLA I类同种异体特异性没有检测到黑色素瘤细胞与单态决定簇的HLA I类抗原的单克隆抗体染色。后一种试剂已在大多数已发表的研究中用于表征黑素瘤病变中HLA I类抗原的表达。此外，本研究结果提供了一种机制，尽管通过用单形决定簇的mAb染色黑色素瘤细胞测得HLA I类抗原的高表达，但在一些患者中对细胞毒性T细胞介导的裂解的意外抗性和疾病的意外不良临床病程。
Immunoperoxidase staining of frozen sections from surgically removed melanoma lesions showed that anti-human leukocyte antigen (HLA)-A2, A28 monoclonal antibody (mAb) stained keratinocytes, but did not stain melanoma cells in 21% of the 14 primary and 44% of the 9 metastatic lesions tested. The loss of HLA-A2 and/or A28 allospecificities did not affect the staining patterns with mAb recognizing monomorphic determinants of HLA Class I antigens, in terms of percentage of stained melanoma cells and intensity of staining. This finding is not likely to reflect the sensitivity of the immunoperoxidase technique, since cytofluorographic analysis detected no significant difference in the staining pattern by mAb to monomorphic determinants of HLA Class I antigens between a melanoma cell line and an autologous transfectant that had acquired HLA-A2 antigens following gene transfer. The results of the present study imply that the frequency of abnormalities in HLA Class I antigen expression by melanoma cells is higher than that described in the literature, since selective losses of HLA Class I allospecificities are not detected by staining of melanoma cells with mAb to monomorphic determinants of HLA Class I antigens. The latter reagents have been used in most of the published studies to characterize the expression of HLA Class I antigens in melanoma lesions. Furthermore, the present results provide a mechanism for the unexpected resistance to cytotoxic T-cell-mediated lysis and the unexpected poor clinical course of the disease in some patients despite a high expression of HLA Class I antigens as measured by staining of melanoma cells with mAb to monomorphic determinants.