An efficient method to screen for the soil bacteria producing therapeutically effective antibiotics
An efficient method to screen for the soil bacteria producing therapeutically effective antibiotics
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DOI：
10.1038/s41429-021-00476-5
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发表时间：
2021-09
期刊：
影响因子：
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通讯作者：
H. Hamamoto;Suresh Panthee;Kana Hashimoto;T. Tsuchida;K. Sekimizu
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文献类型：
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作者：
H. Hamamoto;Suresh Panthee;Kana Hashimoto;T. Tsuchida;K. Sekimizu
新型治疗性抗菌药物的发现已成为应对多重耐药细菌传播的全球危机的紧迫问题。在这份报告中，我们提出了一个有效的筛选方法，从土壤细菌的治疗潜力的抗菌药物。使用这种方法，首先在仅含有土壤提取物的琼脂平板上形成土壤细菌的菌落，然后覆盖含有病原体（抗生素靶标）的软琼脂。然后，我们选择在软琼脂上形成抑制区的菌落，并使用家蚕细菌感染模型评估其培养上清液的治疗效果。使用金黄色葡萄球菌作为指示菌株来获得产生治疗有效的抗菌剂的细菌，与传统方法相比，我们成功地将筛选大小减少了20倍。对本研究中鉴定的86种抗生素生产者的分析表明，大多数属于链霉素。和溶藻属，著名的次级代谢产物生产商。此外，在所鉴定的种类中，有8属37种抗生素产生菌，显示了抗生素产生菌的多样性。基于我们的研究结果，我们提出这种方法作为一种有效的方法来发现新的抗菌剂，是治疗有效的。
The discovery of novel therapeutic antimicrobials has become an urgent issue in response to the global crisis of the spread of multi-drug-resistant bacteria. In this report, we propose an efficient screening method for antimicrobial agents with therapeutic potential from soil bacteria. With this method, colonies of the soil bacteria were formed first on agar plates containing only an extract of soil, followed by an overlay of soft agar containing the pathogens, an antibiotic target. Then, we selected the colonies that formed the inhibitory zones on soft agar and evaluated the therapeutic efficacy of their culture supernatants using a silkworm bacterial infection model. UsingStaphylococcus aureusas an indicator strain to obtain bacteria that produce therapeutically effective antimicrobials, we succeeded in reducing the screening size by 20-fold compared to the conventional method. An analysis of 86 antibiotics producers identified in this study indicated that the majority belonged toStreptomycessp. andLysobactersp., well-known producers of secondary metabolites. Besides, the presence of eight genera and 37 species among the identified species indicated the diversity of antibiotic producers. Based on the finding of our study, we propose this method as an efficient way to discover novel antimicrobial agents that are therapeutically effective.