PREOPTIC-HYPOTHALAMIC PERIVENTRICULAR LESIONS REDUCE NATRIURESIS TO VOLUME EXPANSION
PREOPTIC-HYPOTHALAMIC PERIVENTRICULAR LESIONS REDUCE NATRIURESIS TO VOLUME EXPANSION
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DOI：
10.1152/ajpregu.1983.244.1.r51
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发表时间：
1983-01-01
影响因子：
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通讯作者：
JOHNSON, AK
中科院分区：
文献类型：
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作者：
BEALER, SL;HAYWOOD, JR;JOHNSON, AK
本实验旨在确定围绕腹侧第三脑室（AV3V）的心室周围组织的电解消融是否改变了等渗容量扩张期间典型的尿钠现象。对照组和av3v损伤大鼠以0.5 ml/min的速度静脉滴注0.9% NaCl，直至给予10%体wt。在整个实验过程中监测动脉血压，并收集尿液。膨胀后，血液经处理，通过色谱分离血浆提取物分析利钠激素样活性，然后通过分离的蟾蜍膀胱测量抗血小板活性。AV3V区周围病变大鼠扩张时尿钠排泄量和尿量明显少于对照组。蟾蜍膀胱生物测定显示，容量扩张后的对照动物血浆中钠素样活性较高，而AV3V病变的容量扩张大鼠血浆中没有钠素样活性。AV3V心室周围消融术可减轻等容扩张引起的尿钠。初步结果表明，AV3V区域可能是利钠激素样活性和细胞外液量控制的关键中心部位。
The present experiment was designed to determine if electrolytic ablation of the periventricular tissue surrounding the anteroventral third ventricle (AV3V) altered the natriuresis typically seen during isotonic volume expansion. Control and AV3V-lesioned rats received i.v. infusions of 0.9% NaCl at 0.5 ml/min until 10% body wt was given. Arterial blood pressure was monitored, and urine was collected throughout the experiment. Following expansion, blood was processed for analysis of natriuretic hormone-like activity by chromatographic separation of plasma extracts followed by measuring antinatriferic activity across the isolated toad bladder. Urinary Na excretion and urine volume during expansion were significantly less in rats with lesions surrounding the AV3V region than in control rats. Toad bladder bioassay showed a high level of natriuretic hormone-like activity in control animals following volume expansion, but no natriuretic hormone-like activity in plasma from volume-expanded rats with AV3V lesions. AV3V periventricular ablation attenuates the natriuresis induced by isotonic-volume expansion. Preliminary results indicate the AV3V region may be a central site critical for natriuretic hormone-like activity and control of extracellular fluid volume.