One-pot electrodeposition of 3-aminopropyltriethoxysilane-chitosan hybrid gel film to immobilize glucose oxidase for biosensing
One-pot electrodeposition of 3-aminopropyltriethoxysilane-chitosan hybrid gel film to immobilize glucose oxidase for biosensing
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一锅电沉积3-氨基丙基三乙氧基硅烷-壳聚糖杂化凝胶膜固定化葡萄糖氧化酶用于生物传感
DOI：
10.1016/j.snb.2011.03.063
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发表时间：
2011-09-20
影响因子：
8.4
通讯作者：
Yao, Shouzhuo
中科院分区：
文献类型：
--
作者：
Lei, Lihong;Cao, Zhijun;Yao, Shouzhuo
本文报道了在Au或镀铂Au（Pt（nano）/Au）电极上电沉积3-氨丙基三乙氧基硅烷-壳聚糖（APTES-CS）杂化凝胶膜原位固定葡萄糖氧化酶（GOx）的方法。对苯醌的可控电还原被用来提升GOx-APTES-CS共沉积的电极表面pH，这是由电化学石英晶体微天平监测。优化了传感器的制备工艺和影响传感性能的参数。所制备的GOx-APTES-CS/Pt（nano）/Au和GOx-APTES-CS/Au多孔电极分别在0.5和0.7 V vsSCE下可用于检测酶促生成的H2 O2。该酶电极对葡萄糖浓度在0.2 μ M ~ 8.2mM范围内呈线性响应（R = 0.998，在Pt（纳米）/Au基底）和0.2 μ M至5.5 mM（R = 0.998，在Au衬底），电流灵敏度分别为69.5（Pt（纳米）/Au）和65（Au）μ A mM（-1）cm（-2），每个电极的检测限为0.2 μ M（S/N = 3）。响应时间小于5（Pt（纳米）/Au）或8（Au）s。令人惊讶的是，酶电极在4 ℃下在0.10 M PBS（pH 7.0）中储存5个月（Au）和>6个月（Pt（纳米）/Au）后保持其初始响应灵敏度。(C)2011 Elsevier B. V.保留所有权利。
We report on the electrodeposition of a 3-aminopropyltriethoxysilane-chitosan (APTES-CS) hybrid gel film for in situ immobilization of glucose oxidase (GOx) on an Au or platinized Au (Pt(nano)/Au) electrode for biosensing of glucose. Controllable electroreduction of p-benzoquinone is used to lift the electrode-surface pH for the GOx-APTES-CS codeposition, which was monitored by an electrochemical quartz crystal microbalance. The fabrication procedures of the biosensor and the parameters influencing the biosensing performance were optimized. The prepared porous GOx-APTES-CS/Pt(nano)/Au and GOx-APTES-CS/Au electrodes can be used to detect the enzymatically generated H(2)O(2) at 0.5 and 0.7 V vs SCE, respectively. The enzyme electrodes exhibited linear responses to glucose concentration from 0.2 mu M to 8.2 mM (R = 0.998, at Pt(nano)/Au substrate) and from 0.2 mu M to 5.5 mM (R = 0.998, at Au substrate), with current sensitivities of 69.5 (Pt(nano)/Au) and 65 (Au) mu A mM(-1) cm(-2), respectively, and a detection limit of 0.2 mu M (S/N = 3) was achieved for each electrode. The response time was less than 5 (Pt(nano)/Au) or 8 (Au) s. It is striking that the enzyme electrodes remained their initial response sensitivity after storage for 5 (Au) and >6 (Pt(nano)/Au) months in 0.10 M PBS (pH 7.0) at 4 degrees C. (C) 2011 Elsevier B.V. All rights reserved.