Poly-l-lysine-based tissue embedding compatible with matrix-assisted laser desorption ionization-mass spectrometry imaging analysis of dry and fragile aristolochia plants.
Poly-l-lysine-based tissue embedding compatible with matrix-assisted laser desorption ionization-mass spectrometry imaging analysis of dry and fragile aristolochia plants.
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DOI：
10.1016/j.chroma.2019.460389
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发表时间：
2019-12
期刊：
影响因子：
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通讯作者：
Yu He;Wenjing Guo;Kailong Luo;Qianqian Sun;Zian Lin;Z. Cai
中科院分区：
文献类型：
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作者：
Yu He;Wenjing Guo;Kailong Luo;Qianqian Sun;Zian Lin;Z. Cai
组织的完整性对于基质辅助激光解吸电离-质谱仪(MALDI-MSI)的高质量分析至关重要。对于干燥和脆弱的植物组织的MALDI-MSI，一般不推荐使用传统组织切片技术中使用的各种包埋介质，因为低质量区域MALDI电离的离子抑制效应以及样品制备过程中不希望看到的结构变形。建立了一种新的基于多L赖氨酸的组织包埋方法，用于马兜铃属植物干燥和易碎组织的MALDI-MSI分析。对干燥、脆弱的AP组织进行固定、包埋、冷冻切片和贴片的实际应用表明，与未包埋和明胶包埋相比，基于PLL的包埋技术可以为植物组织分析提供更好的刚性。在PLL包埋介质的辅助下，MALDI-MSI可以获得AP根组织中主要化合物包括马兜铃酸I(AAI)和马兜铃酸II(AAII)的高空间分辨率的分子离子图谱，信号强度增强，没有明显的背景干扰。这项工作为嵌入干燥和脆弱的植物组织提供了一种可与MALDI-MSI分析相媲美的替代方法。
The integrity of tissue is crucial for a high-quality analysis of matrix-assisted laser desorption ionization-mass spectrometry image (MALDI-MSI). Various embedding media utilized in traditional tissue-sectioning techniques are generally not recommended for MALDI-MSI of dry and fragile plant tissues because of the ion suppression effect in MALDI ionization in the low-mass region as well as the undesirable structural deformation during the sample preparation. In this work, a novel poly-L-lysine (PLL)-based tissue embedding method was developed for MALDI-MSI analysis of dry and fragile aristolochia plant (AP) tissues. The practical application in fixation, embedding, cryosectioning, and mounting of the dry and fragile AP tissues demonstrated that the PLL-based embedding technique could provide good rigidity to the plant tissues analysis compared to that without embedding and gelatin embedding. With the assistance of the PLL embedding medium, high spatial resolution molecular ion maps of main compounds, including aristolochic acids I (AAI) and aristolochic acids II (AAII) in AP root tissue, could be achieved by MALDI-MSI with enhanced signal intensities and no obvious background interference. This work provides an alternative approach for embedding the dry and fragile plant tissues comparable with MALDI-MSI analysis.