The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity
The role of actin in the regulation of dendritic spine morphology and bidirectional synaptic plasticity
复制标题
DOI：
10.1097/00001756-200404090-00018
复制
发表时间：
2004-04-09
期刊：
影响因子：
1.7
通讯作者：
Fitzsimonds, RM
中科院分区：
文献类型：
--
作者：
Chen, YC;Bourne, J;Fitzsimonds, RM
树突棘是哺乳动物中枢神经系统中兴奋性突触的首选部位，是富含肌动蛋白的结构。我们假设棘中肌动蛋白的动态调节会对导致突触功效增强和抑制的过程产生不同的影响。在这里，我们报告说，在大鼠海马切片中肌动蛋白聚合存在的情况下，兴奋性突触传递的长期抑制的表达持续存在。我们观察到高频刺激对LTD（去抑郁）的逆转被完全阻断。使用电子显微镜，观察到树突棘形态的巨大变化，伴随着兴奋性突触传递的持续、不可逆的抑制。
Dendritic spines, which are the preferred site of excitatory synapses in the mammalian CNS, are actin-rich structures. We hypothesized that dynamic regulation of actin in spines would differentially affects processes that lead to potentiation vs depression of synaptic efficacy. Here, we report that the expression of long-term depression of excitatory synaptic transmission persists in the presence of actin polymerization in rat hippocampal slices. We observe that the reversal of LTD, de-depression, by high-frequency stimulation was completely blocked. Using electron microscopy, dramatic changes in dendritic spine morphology which accompany the sustained, irreversible depression of excitatory synaptic transmission were observed.