Structure, organization and regulation of a rat cysteine proteinase inhibitor-encoding gene.
Structure, organization and regulation of a rat cysteine proteinase inhibitor-encoding gene.
复制标题
大鼠半胱氨酸蛋白酶抑制剂编码基因的结构、组织和调控。
DOI：
10.1016/0378-1119(92)90645-6
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发表时间：
1992
期刊：
影响因子：
3.5
通讯作者：
Shaw,PA
中科院分区：
文献类型：
--
作者：
Cox,JL;Shaw,PA
在出生后的发育过程中，大鼠的下颌下腺产生分泌蛋白，半胱氨酸蛋白酶抑制剂S（CysS），它属于哺乳动物半胱氨酸蛋白酶抑制剂超家族的第2家族。虽然ratCysS基因在成年大鼠的唾液腺中不表达，但它可以被异丙肾上腺素（IPR）诱导，其通过β-肾上腺素能受体/腺苷酸环化酶/环AMP（cAMP）机制起作用。此外，在青春期前和成熟年龄，雌性下颌下腺中CysS mRNA的IPR诱导比雄性更明显。这些结果表明，性激素可能通过雌激素反应元件（ERE）参与大鼠CysS基因的调控，该基因的IPR诱导支持cAMP反应元件（CRE）也可能在调控CysS基因表达中起作用的假设。我们已经分离、测序并鉴定了完整的基因。CysS基因包含三个外显子，中间有两个插入序列，具有一致的剪接点。转录起始点位于起始密码子上游73个核苷酸处，该起始密码子由典型的Kozak序列包围。CCAAT和TATA盒存在于CysS基因的5 ′-侧翼区。该区域还包含几个可能的调控元件，类似于其他真核基因，即ERE，CRE和糖皮质激素反应元件。第一个内含子序列含有其他潜在的CREs，与IPR诱导的小鼠和仓鼠富含脯氨酸蛋白编码基因中发现的CREs高度同源。
During postnatal development, submandibular glands of rats produce the secrretory protein, cystatin S (CysS), which belongs to family 2 of the mammalian cysteine proteinase inhibitor superfamily. While the ratCysSgene is not expressed in the salivary glands of adult rats, it can be induced by isoproterenol (IPR), which acts via β-adrenergic receptor/adenylate cyclase/cyclic AMP (cAMP) mechanisms In addition, IPR-induction of CysS mRNA in submandibular glands is more pronounced in females than in males, at both prepuberal and mature ages. These results suggest that sex hormones may participate in the regulation of the ratCysSgene via estrogen-responsive elements (ERE), and IPR induction of this gene supports the hypothesis that cAMP-responsive elements (CRE) may also play a role in regulatingCysSgene expression. We have isolated, sequenced and characterized the complete gene. TheCysSgene contains three exons interrupted by two intervening sequences, with consensus splice junctions. The transcription start point is 73 nucleotides upstream from the start codon which is suurrounded by a typical Kozak sequence. CCAAT and TATA boxes are present in the 5′-flanking region of theCysSgene. This region also contains several possible regulatory elements that resemble those of other eukaryotic genes, i.e.ERE, CRE, and glucocorticoid-responsive elements. The first intron sequence contains other potentialCREhighly homologous to those found in the IPR-inducible mouse and hamster proline-rich-protein-encoding genes.