Characterization of mycoplasma strains isolated from house musk shrews (Suncus murinus) and their infectivity in mice and rats.
Characterization of mycoplasma strains isolated from house musk shrews (Suncus murinus) and their infectivity in mice and rats.
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从家麝鼩 (Suncus murinus) 中分离出的支原体菌株的特征及其对小鼠和大鼠的感染性。
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
R. Harasawa
中科院分区：
文献类型：
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作者：
K. Goto;T. Itoh;S. Ebukuro;N. Kagiyama;R. Harasawa
本文报道了从实验动物中心的家麝中分离到的5株支原体，并与3株小鼠支原体（肺支原体M 53，M. arthritis PG 6和M. A型溶神经毒素，并与先前从家麝中分离的参考菌株G3-5进行比较。这些分离物发酵葡萄糖，但不水解尿素和精氨酸，通过450 nm孔径的膜过滤器，对毛地黄皂苷敏感，并在琼脂培养基上形成微小（直径115至231微米）菌落。所有五个未分类的家麝猴支原体菌株和用作参考的菌株G3-5基于（i）它们的抗原特性构成了一个同质组（使用代谢抑制试验测定），（ii）它们的多肽谱（使用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳和蛋白质印迹法测定），和（iii）它们的基因组特性（使用DNA切割模式分析确定），但与基于这些发现建立的鼠支原体非常不同。在我们的殖民地的56个家麝香鼩年龄2至45周的调查，支原体从所有动物的口腔检查分离。在分离出支原体菌株的5只动物中未观察到肉眼或显微镜下病变。在实验感染中，支原体对小鼠和大鼠无感染性。结果表明，这组支原体是一个共同的居民的家麝。
Five mycoplasma strains isolated from house musk shrews (Suncus murinus) in the Central Institute for Experimental Animals were characterized and compared with three murine mycoplasma strains, Mycoplasma pulmonis m 53, M. arthritidis PG6, and M. neurolyticum Type A, and with reference strain G3-5 previously isolated from a house musk shrew. These isolates fermented glucose, but did not hydrolyze urea and arginine, passed through membrane filters of 450 nm pore size, were sensitive to digitonin, and formed minute (115 to 231 microns in diameter) colonies on agar medium. All the five unclassified house musk shrew mycoplasma strains and strain G3-5 used as a reference constituted a homogeneous group based on (i) their antigenic properties (determined using the metabolism inhibition test), (ii) their polypeptide profiles (determined using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the Western blotting assay), and (iii) their genomic properties (determined using DNA cleavage pattern analysis), but were quite distinct from the established murine mycoplasmas on the basis of these findings. In a survey of 56 house musk shrews aged 2 to 45 weeks in our colonies, mycoplasmas were isolated from the oral cavities of all animals examined. No gross or microscopic lesions were observed in the five animals from which the mycoplasma strains were isolated. In experimental infection, the mycoplasma was not infective for mice and rats. The results suggest that this group of mycoplasmas is a common inhabitant of house musk shrews.
通过酶联免疫吸附测定 (ELISA) 检测大鼠和小鼠中的天然肺支原体感染。
DOI：
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发表时间：
1981
期刊：
Laboratory animal science
影响因子：
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作者：
Cassell,GH;Lindsey,JR;Davis,JK;Davidson,MK;Brown,MB;Mayo,JG
通讯作者：
Mayo,JG