Rapid screening and characterization of metabolites from a marine-derived actinomycete by high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry
Rapid screening and characterization of metabolites from a marine-derived actinomycete by high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry
复制标题
通过高效液相色谱结合电喷雾电离四极杆飞行时间质谱法快速筛选和表征海洋放线菌的代谢物
DOI：
10.1002/rcm.4744
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发表时间：
2010-12-15
影响因子：
2
通讯作者：
Xi, Tao
中科院分区：
文献类型：
--
作者：
Liu, Cheng-Wei;Lu, Yuan-Yuan;Xi, Tao
通过高效液相色谱结合电喷雾电离四极杆飞行时间质谱 (HPLC/QTOF MS/MS) 优化并建立了一种快速可靠的方法，用于分析海洋放线菌的代谢物。从MS/MS谱中，记录了[M+H](+)的产物离子，提供了母核和肽部分的丰富结构信息。使用 QTOF MS/MS 和源内碰撞诱导解离（源内 CID）技术，对包括放线菌素 D、放线菌素 V 和放线菌素 I 在内的三种主要代谢物进行了测定，并通过前体离子和产物离子的元素组成进行了表征（
A rapid and reliable method has been optimized and established for the analysis of the metabolites from a marine actinomycete by high-performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (HPLC/QTOF MS/MS). From MS/MS spectra, the product ions of [M+H](+) were recorded to provide abundant structural information of the mother nucleus and peptide moieties. Using the QTOF MS/MS and in-source collision-induced dissociation (in-source CID) techniques, three main metabolites including actinomycin D, actinomycin V and actinomycin I were determined and characterized by elemental compositions of precursor and product ions (