Localization and phototoxic effect of zinc sulfophthalocyanine photosensitizer in human colon (DLD-1) and lung (A549) carcinoma cells (in vitro)
Localization and phototoxic effect of zinc sulfophthalocyanine photosensitizer in human colon (DLD-1) and lung (A549) carcinoma cells (in vitro)
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DOI：
10.1016/j.pdpdt.2011.08.006
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发表时间：
2012-03-01
影响因子：
3.3
通讯作者：
Abrahamse, Heidi
中科院分区：
文献类型：
--
作者：
Manoto, Sello Lebohang;Sekhejane, Palesa Rose;Abrahamse, Heidi
背景：光动力疗法（PDT）是一种用于治疗癌细胞的治疗方式。先前已经显示，混合磺化金属酞菁络合物，磺基酞菁锌（ZnPcSmix）在破坏肺癌细胞方面是有效的。方法：ZnPcSmix以5J/cm（2）的能量密度在680nm波长下激活，观察ZnPcSmix的亚细胞定位及其对两种肿瘤细胞的作用。使用结肠（DLD-1）和肺（A549）癌细胞系。通过荧光显微镜确定ZnPcSmix的亚细胞定位。通过细胞形态、活力、增殖和细胞毒性测定光动力疗法（PDT）单独和光与PS联合作用的毒性。结果：ZnPcSmix在A549细胞和DLD-1细胞中均定位于溶酶体和线粒体中，并与未照射（0J/cm（2））、单纯照射（5J/cm（2））和单纯PS处理（未照射）的细胞作对照。用PDT处理的A549细胞在所有使用的PS浓度下显示出存活力和增殖的显著降低，而在DLD-1细胞中，在10、20和40 μ M的浓度下观察到显著降低。在没有光的情况下，ZnPcSmix没有导致细胞毒性在A549细胞，而在DLD-1细胞中，它导致细胞增殖的减少，只有在40 μ M的浓度。结论：ZnPcSmix是有效的诱导细胞死亡，在这两个细胞系定位在重要的细胞器，如线粒体和溶酶体，这是细胞功能所必需的。光活化ZnPcSmix在不同浓度下对细胞有不同程度的作用，并在体外获得了良好的治疗效果。(c)2011爱思唯尔有限公司版权所有。
Background: Photodynamic therapy (PDT) is a therapeutic modality used for treating cancerous cells. It has been previously shown that mixed sulfonated metallophthalocyanine complex, zinc sulfophthalocyanine (ZnPcSmix) is effective in destroying lung cancer cells. This study aimed to determine subcellular localization of ZnPcSmix and its effect on two cancer cell lines.Methods: ZnPcSmix was activated at a wavelength of 680 nm with 5 J/cm(2). Colon (DLD-1) and lung (A549) cancer cell lines were used. Subcellular localization of ZnPcSmix was determined by fluorescence microscopy. Toxicity of PS alone and combination of light and PS (PDT) was determined by cell morphology, viability, proliferation and cytotoxicity. Cells which received no irradiation (0 J/cm(2)), irradiation alone (5 J/cm(2)) or treated with PS alone (no irradiation) served as controls.Results: ZnPcSmix localized in both lysozomes and mitochondria in both A549 and DLD-1 cells. A549 cells treated with PDT showed a significant decrease in viability and proliferation in all PS concentrations used, while in DLD-1 cells a significant decrease was seen with concentrations of 10, 20 and 40 mu M. In absence of light, ZnPcSmix did not result in cellular toxicity in A549 cells whereas in DLD-1 cells it resulted in a reduction in cell proliferation only at a concentration of 40 mu M.Conclusion: ZnPcSmix was effective in inducing cell death in both cell lines when localized in vital organelles such mitochondria and lysozomes which are essential for cell functioning. Photoactivated ZnPcSmix affected the cells at different concentration and yielded good therapeutic results in vitro. (c) 2011 Elsevier B.V. All rights reserved.