Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain.
Activation of diacylglycerol in cultured endothelial cells exposed to cyclic strain.
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暴露于循环应变的培养内皮细胞中二酰甘油的激活。
DOI：
10.1152/ajpcell.1997.272.2.c650
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Sumpio,BE
中科院分区：
文献类型：
--
作者：
Evans,L;Frenkel,L;Brophy,CM;Rosales,O;Sudhaker,CB;Li,G;Du,W;Sumpio,BE
Confluent bovine aortic endothelial cells (EC) were grown on flexible membranes and subjected to 10% average strain at 60 cycles/min for up to 500 s. A biphasic increase in diacylglycerol (DAG) occurred, with an initial transient peak at 10 s followed by sustained elevation to 500 s. The early peak corresponded to the transient formation of inositol 1,4,5-trisphosphate, demonstrating hydrolysis of L-alpha-phosphatidylinositol (PI) by PI-specific phospholipase C. To determine the origin of the sustained DAG phase, we incubated confluent bovine aortic EC with 1 microCi/ml [14C]myristate overnight and subjected them to cyclic strain. There was a decrease in phosphatidylcholine (PC) and a corresponding increase in DAG at 10 s and 250 s, suggesting PC hydrolysis with the generation of DAG at both an early (10 s) and a late (250 s) phase. [14C]phosphatidylethanol, a specific product of phospholipase D (PLD) in the presence of 1% ethanol, was measured in EC preincubated with [14C]myristate. Cyclic strain led to an immediate and sustained activation of PLD. Increased ethanol concentration led to a consistent decrease in DAG. Furthermore, when EC were pretreated with 1% ethanol, the strain-induced proliferative response was attenuated.
